The sialoadhesin (CD169) expressing a macrophage subset in human proliferative glomerulonephritis
| Autor: | Shinichi Hayafuji, Makoto Uchiyama, Hiroshi Kawachi, Soichiro Okubo, Fujio Shimizu, David J. Nikolic-Paterson, Yohei Ikezumi, Toshiaki Suzuki |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Thin basement membrane disease Pathology medicine.medical_specialty Adolescent Glomerulonephritis Membranoproliferative Sialic Acid Binding Ig-like Lectin 1 Biopsy Kidney Glomerulus Lupus nephritis Severity of Illness Index Nephropathy Antigens CD Sialoadhesin Membranoproliferative glomerulonephritis medicine Humans Receptors Immunologic Child Fluorescent Antibody Technique Indirect Transplantation Membrane Glycoproteins business.industry Macrophages Glomerulonephritis medicine.disease Nephrology Female business Nephritis Kidney disease |
| Zdroj: | Nephrology Dialysis Transplantation. 20:2704-2713 |
| ISSN: | 1460-2385 0931-0509 |
| Popis: | Background. Sialoadhesin (Sn; CD169) is a lectinlike receptor whose expression is restricted to subsets of tissue and inflammatory macrophages. We have previously identified accumulation of Snþ macrophages as an important marker of disease progression versus remission in rat mesangial proliferative nephritis. The current study examined the significance of Snþ macrophages in human proliferative glomerulonephritis. Methods. Frozen kidney sections from normal adult human kidney (n ¼ 4) and pediatric nephropathy (n ¼ 40) were stained for total macrophages (CD68þ cells), Snþ macrophages, CD3þ T-cells and collagen type I by immunofluorescence. Leukocyte infiltration and the severity of glomerular lesions and interstitial damage were scored. A second protocol biopsy was performed in 27 cases and clinical and biopsy-based data obtained. Results. Snþ macrophages were absent from glomeruli in normal adult human kidney and in thin basement membrane disease (n ¼ 4), but were detected in 4 of 9 cases of purpura nephritis; 7 of 17 IgA nephropathy; 5 of 5 membranoproliferative glomerulonephritis, and 5 of 5 lupus nephritis. Snþ macrophages were localized in areas of focal glomerular and interstitial damage. Two-colour immunostaining confirmed that Snþ cells are a subset of total CD68þ macrophages. The number of glomerular Snþ macrophages correlated with the degree of proteinuria and glomerular lesions (r ¼ 0.44, P ¼ 0.0045 and r ¼ 0.82, P |
| Databáze: | OpenAIRE |
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