| Autor: |
William W. Lin, Jacki Abrams, Larry I. Lipshultz, Edward D. Kim |
| Rok vydání: |
1997 |
| Předmět: |
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| Zdroj: |
The Journal of Urology. :147-150 |
| ISSN: |
0022-5347 |
| DOI: |
10.1097/00005392-199701000-00047 |
| Popis: |
Purpose: Evaluation of the testis biopsy specimen in male infertility has been hampered by the qualitative rather than quantitative nature of routine histological techniques and interpretation. Although useful, flow cytometry has demonstrated significant limitations due to its inability to differentiate among haploid cell types, spermatozoa and spermatids. Thus, deoxyribonucleic acid image analysis was performed on testis biopsy specimens to combine the advantages of quantitative ploidy and morphological characteristics to develop a more effective quantitative system.Materials and Methods: Image analysis using a specifically designed filter was performed on Feulgen stained 5 micro m. sections of paraffin embedded testicular tissue. The archival testicular tissue had been obtained using standard biopsy techniques from patients with azoospermia and normal spermatogenesis on routine hematoxylin and eosin processed tissue.Results: The overall findings (mean plus or minus standard deviation) in 20 biop... |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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