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Betty Agustina Tambunan,1â 3 I Dewa Gede Ugrasena,3,4 Aryati2,3 1Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; 2Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; 3Dr. Soetomo General Academic Hospital, Surabaya, Indonesia; 4Department of Pediatrics, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaCorrespondence: Aryati, Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, St. Mayjen. Prof. Dr. Moestopo 47, Surabaya, East Java, 60132, Indonesia, Tel +6281230570493 ; +62-031-5020251, Email aryati@fk.unair.ac.idIntroduction: Repeated transfusions in thalassemia patients can cause several complications, including alloimmunization and autoimmunization.Purpose: This study compares the immune response of T follicular helper (Tfh) lymphocytes expressing T-cell immunoreceptor with immunoglobulin and immunoreceptor tyrosine-based inhibitory domains (TIGIT), programmed cell death-1 (PD-1), and interleukin-21 (IL-21) between patients with allo-auto positive and negative thalassemia before and after hemin administration.Materials and Methods: This study used a quasi-experimental pre- and post-test design and was performed between April and November 2021 at the Dr. Soetomo General Academic Hospital in Surabaya, Indonesia. It enrolled 29 patients with allo-auto positive thalassemia and 28 with allo-auto negative, and 9 mL of whole blood (WB) was drawn from each patient. Hemin solution (20 μM) was added to 5 mL of WB, incubated for two hours, processed into peripheral blood mononuclear cells (PBMCs) in RPMI media, and cultured with 5% CO2 for three days. The 4 mL WB sample was also processed into PBMCs. PBMC cells cultured and without cultured were examined by flow cytometry using a BD FACSCalibur after surface and intracellular staining. Differences in Tfh cells expressing TIGIT, PD-1, and IL-21 between thalassemia groups before and after hemin administration were compared using independent t-tests or MannâWhitney U-tests (p < 0.05).Results: Tfh cell expression did not differ between groups before hemin administration and increased after hemin administration. The increase in Tfh cell expression was higher in the allo-auto positive group. TIGIT and PD-1 expression in Tfh cells did not differ between groups, but TIGIT decreased after hemin administration in contrast to PD-1 result. IL-21 expression in Tfh cells did not differ between groups and did not change after hemin administration.Conclusion: Hemin affected the expression of Tfh cells in both group thalassemia, but there was no difference of Tfh cell expression between the groups.Keywords: thalassemia disease, T follicular helper, TIGIT, PD-1, interleukin-21 |
