Comparison of Two Methods for Counting Molds in Fermentations Using the Production of Bikaverin by Fusarium oxysporum CCT7620 as a Model
| Autor: | Marcela Colombo dos Santos, Bianca Ferreira da Silva, Wesley Santiago da Silva, Marcel Otavio Cerri, Marcelo Perencin de Arruda Ribeiro, Juliano Lemos Bicas |
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| Přispěvatelé: | Universidade Estadual de Campinas (UNICAMP), Federal University of São João Del-Rei. Ouro Branco, Universidade Estadual Paulista (Unesp), Universidade Federal de São Carlos (UFSCar) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Fusarium
0303 health sciences animal structures biology Serial dilution 030306 microbiology Standard plate fungi Airlift Biomass General Medicine biology.organism_classification Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Fusarium oxysporum Bioreactor Fermentation Food science 030304 developmental biology |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2020-12-12T01:36:05Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 The dry cell weight (DCW) measurement is one of the preferred methods to determine the growth of filamentous fungi. However, this technique is not applicable to insoluble culture media, besides being possibly influenced by the presence of extracellular biomass. The standard plate counting (SPC) is a reference method for detecting viable cells; however, it is referred as imprecise. In this study, we did a comprehensive analysis of the errors associated to each procedure and also determined the growth kinetics of Fusarium oxysporum in soluble (DCW and SPC) and insoluble (SPC) culture media. Finally, we used the production of bikaverin in airlift bioreactor containing insoluble medium as a case study to estimate red pigment production and to monitor biomass growth via SPC. We concluded that SPC can be used to give reliable fungal growth kinetics in media with insoluble matter, yielding errors equivalent to DCW depending on the number of replicates done for serial dilutions and plate counting. Faculdade de Engenharia de Alimentos Department of Food Science University of Campinas, Rua Monteiro Lobato, 80 Federal University of São João Del-Rei. Ouro Branco, Campus Alto Paraopeba Department of Analytical Chemistry UNESP Universidade Estadual Paulista Department of Bioprocess and Biotechnology UNESP Universidade Estadual Paulista Chemical Engineering Department Federal University of São Carlos UFSCar Department of Analytical Chemistry UNESP Universidade Estadual Paulista Department of Bioprocess and Biotechnology UNESP Universidade Estadual Paulista |
| Databáze: | OpenAIRE |
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