Injection of air into the headspace improves fermentation of phosphoric acid pretreated sugarcane bagasse by Escherichia coli MM170

Autor: Ismael U. Nieves, Lonnie O. Ingram, M.T. Mullinnix, Zhaohui Tong, C.C. Geddes, R.W. Hoffman, Eulogio Castro, Keelnatham T. Shanmugam
Rok vydání: 2011
Předmět:
Zdroj: Bioresource Technology. 102:6959-6965
ISSN: 0960-8524
DOI: 10.1016/j.biortech.2011.04.036
Popis: Microaeration (injecting air into the headspace) improved the fermentation of hemicellulose hydrolysates obtained from the phosphoric acid pretreatment of sugarcane bagasse at 170 °C for 10 min. In addition, with 10% slurries of phosphoric acid pretreated bagasse (180 °C, 10 min), air injection into the headspace promoted xylose utilization and increased ethanol yields from 0.16 to 0.20 g ethanol/g bagasse dry weight using a liquefaction plus simultaneous saccharification and co-fermentation process (L+SScF). This process was scaled up to 80 L using slurries of acid pretreated bagasse (96 h incubation; 0.6 L of air/min into the headspace) with ethanol yields of 312–347 L (82–92 gal) per tonne (dry matter), corresponding to 0.25 and 0.27 g/g bagasse (dry weight). Injection of small amounts of air into the headspace may provide a convenient alternative to subsurface sparging that avoids problems of foaming, sparger hygiene, flotation of particulates, and phase separation.
Databáze: OpenAIRE