Injection of air into the headspace improves fermentation of phosphoric acid pretreated sugarcane bagasse by Escherichia coli MM170
Autor: | Ismael U. Nieves, Lonnie O. Ingram, M.T. Mullinnix, Zhaohui Tong, C.C. Geddes, R.W. Hoffman, Eulogio Castro, Keelnatham T. Shanmugam |
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Rok vydání: | 2011 |
Předmět: |
Environmental Engineering
Carbohydrates Bioengineering Xylose chemistry.chemical_compound Hydrolysis Bioreactors Escherichia coli Sulfites Phosphoric Acids Hemicellulose Dry matter Cellulose Waste Management and Disposal Phosphoric acid Ethanol Chromatography Waste management Renewable Energy Sustainability and the Environment Air General Medicine Saccharum Steam chemistry Fermentation Bagasse Biotechnology |
Zdroj: | Bioresource Technology. 102:6959-6965 |
ISSN: | 0960-8524 |
DOI: | 10.1016/j.biortech.2011.04.036 |
Popis: | Microaeration (injecting air into the headspace) improved the fermentation of hemicellulose hydrolysates obtained from the phosphoric acid pretreatment of sugarcane bagasse at 170 °C for 10 min. In addition, with 10% slurries of phosphoric acid pretreated bagasse (180 °C, 10 min), air injection into the headspace promoted xylose utilization and increased ethanol yields from 0.16 to 0.20 g ethanol/g bagasse dry weight using a liquefaction plus simultaneous saccharification and co-fermentation process (L+SScF). This process was scaled up to 80 L using slurries of acid pretreated bagasse (96 h incubation; 0.6 L of air/min into the headspace) with ethanol yields of 312–347 L (82–92 gal) per tonne (dry matter), corresponding to 0.25 and 0.27 g/g bagasse (dry weight). Injection of small amounts of air into the headspace may provide a convenient alternative to subsurface sparging that avoids problems of foaming, sparger hygiene, flotation of particulates, and phase separation. |
Databáze: | OpenAIRE |
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