BMP7 overexpression in adipose tissue induces white adipogenesis and improves insulin sensitivity in ob/ob mice
| Autor: | Sylvie Franckhauser, Victor Sacristan, Estefania Casana, Cristina Mallol, Xavier León, Miquel Garcia, Claudia Jambrina, Sergio Muñoz, Veronica Jimenez, Fatima Bosch, Jordi Rodó |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
animal structures Endocrinology Diabetes and Metabolism SDG 3 - Good health and well-being Medicine (miscellaneous) Adipose tissue 030209 endocrinology & metabolism White adipose tissue Biology 03 medical and health sciences Paracrine signalling chemistry.chemical_compound 0302 clinical medicine Insulin resistance Internal medicine Adipocyte medicine 030212 general & internal medicine Autocrine signalling Nutrition and Dietetics Endocrinology diabetes and metabolism food and beverages medicine.disease Nutrition and dietetics Endocrinology chemistry Adipogenesis embryonic structures Steatosis hormones hormone substitutes and hormone antagonists |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona |
| Popis: | Altres ajuts: ICREA Academia Award Background/objectives: During obesity, hypertrophic enlargement of white adipose tissue (WAT) promotes ectopic lipid deposition and development of insulin resistance. In contrast, WAT hyperplasia is associated with preservation of insulin sensitivity. The complex network of factors that regulates white adipogenesis is not fully understood. Bone morphogenic protein 7 (BMP7) can induce brown adipogenesis, but its role on white adipogenesis remains to be elucidated. Here, we assessed BMP7-mediated effects on white adipogenesis in ob/ob mice. Methods: BMP7 was overexpressed in either WAT or liver of ob/ob mice using adeno-associated viral (AAV) vectors. Analysis of gene expression, histological and morphometric alterations, and metabolites and hormones concentrations were carried out. Results: Overexpression of BMP7 in adipocytes of subcutaneous and visceral WAT increased fat mass, the proportion of small-size adipocytes and the expression of adipogenic and mature adipocyte genes, suggesting induction of adipogenesis irrespective of fat depot. These changes were associated with reduced hepatic steatosis and improved insulin sensitivity. In contrast, liver-specific overproduction of BMP7 did not promote WAT hyperplasia despite BMP7 circulating levels were similar to those achieved after genetic engineering of WAT. Conclusions: This study unravels a new autocrine/paracrine role of BMP7 on white adipogenesis and highlights that BMP7 may modulate WAT plasticity and increase insulin sensitivity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|