Ultra-thin-layer chromatography mass spectrometry and thin-layer chromatography mass spectrometry of single peptides of angiotensin-converting enzyme inhibitors
| Autor: | Breda Simonovska, Gordana Popović, Irena Vovk, Danica Agbaba, Alen Albreht |
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| Rok vydání: | 2011 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Electrospray TLC Angiotensin-Converting Enzyme Inhibitors 010402 general chemistry Mass spectrometry TLC-MS Prilates 01 natural sciences Biochemistry Image analysis Analytical Chemistry chemistry.chemical_compound Ramipril Lisinopril Tetrahydroisoquinolines Image Processing Computer-Assisted Chromatography Chemistry Silica gel UTLC 010401 analytical chemistry Organic Chemistry Extraction (chemistry) Quinapril General Medicine Cilazapril Thin-layer chromatography Prils 0104 chemical sciences Chromatography Thin Layer Absorption (chemistry) UTLC-MS Peptides Densitometry Layer (electronics) |
| Zdroj: | Journal of Chromatography A |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2011.03.039 |
| Popis: | The separation of structurally related angiotensin-converting enzyme (ACE) inhibitors lisinopril, cilazapril, ramipril and quinapril and their corresponding active diacid forms (prilates) by conventional TLC silica gel 60 plates was contrasted with that afforded by monolithic ultra-thin-layer chromatographic (UTLC) plates. For the use of UTLC plates technical modifications of the commercially available equipments for the sample application, development and detection were made. Plates were developed in modified horizontal developing chamber using ethyl acetate-acetone-acetic acid-water (4:1:0.25:0.5, v/v). Detection of the separated compounds was performed densitometrically in absorption/reflectance mode at 220 nm and after exposure to iodine also by image analysis. The obtained results showed that monolithic layer is more efficient for the separation of structurally similar polar compounds, such as prilates than conventional silica layers. Identification of the compounds was confirmed by ESI-MS after their on-line extraction from the UTLC and TLC plates by means of Camag TLC-MS interface. |
| Databáze: | OpenAIRE |
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