Surgical Maneuvers Enhance Molecular Detection of Circulating Tumor Cells During Gastric Cancer Surgery
| Autor: | Sonshin Takao, Takashi Aikou, Yoshito Eizuru, Koki Tokuda, Fumio Kijima, Shuichi Hokita, Kuniaki Aridome, Shoji Natsugoe, Futoshi Miyazono, Masamichi Baba |
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| Rok vydání: | 2001 |
| Předmět: |
Male
CA15-3 Pathology medicine.medical_specialty Metastasis Circulating tumor cell Breast cancer Stomach Neoplasms medicine Hepatectomy Humans RNA Messenger Aged Reverse Transcriptase Polymerase Chain Reaction business.industry Cancer Original Articles Middle Aged Neoplastic Cells Circulating medicine.disease Primary tumor Carcinoembryonic Antigen Early Gastric Cancer Cancer cell Female Surgery business |
| Zdroj: | Annals of Surgery. 233:189-194 |
| ISSN: | 0003-4932 |
| Popis: | Blood-borne metastasis, including liver metastasis, is one of several important prognostic factors in patients with gastric cancer undergoing curative resection. 1,2 Even in patients with early gastric cancer, blood-borne metastasis occurs. 3–5 In animal studies, cancer cells have been observed to shed into the bloodstream during surgical manipulation of a primary tumor. 6,7 In turn, this shedding of cancer cells into the bloodstream enhanced the distant metastasis. 8,9 Circulating cancer cells are now easily detectable with polymerase chain reaction (PCR) or reverse transcriptase–polymerase chain reaction (RT-PCR). There have been several studies on the detection of circulating cancer cells in the blood by PCR or RT-PCR. 10–15 Eschwege et al 16 showed the dissemination of prostatic epithelial cells during radical prostatectomy in patients with prostatic cancer by RT-PCR. Brown et al 17 detected tumor cell dissemination during surgery in patients with breast cancer using RT-PCR. However, relatively few reports have examined the presence of cancer cells in blood samples from various sites or investigated the relation between the detection of circulation of cancer cells and blood-borne metastases. Gerhard et al 18 established carcinoembryonic antigen (CEA)-specific RT-PCR in 1994. Mori et al 19 detected circulating cancer cells in patients with gastric cancer by CEA-specific RT-PCR. Funaki et al 20 detected CEA-mRNA in the circulating peripheral blood in patients with gastric cancer using their own CEA-specific RT-PCR method. Thus, the CEA-specific RT-PCR method is useful for detecting circulating cancer cells in patients with gastric cancer. We performed CEA-specific RT-PCR on blood samples from several vessels to investigate the relation between the detection of circulating cancer cells during surgery and blood-borne metastases. Thus, the present study examines circulating cancer cells from various sites according to the time course during a surgical procedure and attempts to determine the relation between the appearance of cancer cells in blood as a result of surgical maneuvers and liver metastasis in patients with gastric cancer. |
| Databáze: | OpenAIRE |
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