Bio-hybrid photoconverter by covalent functionalization of the photosynthetic reaction center of Rhodobacter sphaeroides with fluorescein isothiocyanate
Autor: | Rocco Roberto Tangorra, Alessandra Antonucci, Alessandra Operamolla, Roberta Ragni, Francesco Milano, Massimo Trotta, Angela Agostiano, Simona la Gatta, Gianluca M. Farinola |
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Rok vydání: | 2015 |
Předmět: | |
Zdroj: | MRS Proceedings. 1722 |
ISSN: | 1946-4274 0272-9172 |
DOI: | 10.1557/opl.2015.602 |
Popis: | Organic biological hybrid systems, accessible by covalent functionalization of photosynthetic proteins with molecular antennas, represent a promising novelty to enhance natural photosynthesis. In this paper, we present the successful bioconjugation of a commercial fluorophore, fluorescein isothiocyanate (FITC), to the photosynthetic reaction center RC from the bacterium Rhodobacter sphaeroides strain R26. The resulting hybrid outperforms the pristine protein in hole-electron couple generation yield, exclusively at wavelengths where the fluorophore absorbs while the protein does not. |
Databáze: | OpenAIRE |
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