Bio-hybrid photoconverter by covalent functionalization of the photosynthetic reaction center of Rhodobacter sphaeroides with fluorescein isothiocyanate

Autor: Rocco Roberto Tangorra, Alessandra Antonucci, Alessandra Operamolla, Roberta Ragni, Francesco Milano, Massimo Trotta, Angela Agostiano, Simona la Gatta, Gianluca M. Farinola
Rok vydání: 2015
Předmět:
Zdroj: MRS Proceedings. 1722
ISSN: 1946-4274
0272-9172
DOI: 10.1557/opl.2015.602
Popis: Organic biological hybrid systems, accessible by covalent functionalization of photosynthetic proteins with molecular antennas, represent a promising novelty to enhance natural photosynthesis. In this paper, we present the successful bioconjugation of a commercial fluorophore, fluorescein isothiocyanate (FITC), to the photosynthetic reaction center RC from the bacterium Rhodobacter sphaeroides strain R26. The resulting hybrid outperforms the pristine protein in hole-electron couple generation yield, exclusively at wavelengths where the fluorophore absorbs while the protein does not.
Databáze: OpenAIRE