Functionalization of poly(lactic acid) scaffold surface by aminolysis and hyaluronan immobilization: How it affects mesenchymal stem cell proliferation
| Autor: | Brigitte Deschrevel, Alexandre Monnier, Kateryna Fatyeyeva, Jean-Marc Valleton, Elias Al Tawil, Quang Trong Nguyen |
|---|---|
| Přispěvatelé: | Polymères Biopolymères Surfaces (PBS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Scaffold Polymers and Plastics Chemistry Organic Chemistry General Physics and Astronomy Biomaterial 02 engineering and technology [CHIM.MATE]Chemical Sciences/Material chemistry 021001 nanoscience & nanotechnology Lactic acid Glycosaminoglycan 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Aminolysis [CHIM.POLY]Chemical Sciences/Polymers Tissue engineering [CHIM.ANAL]Chemical Sciences/Analytical chemistry Materials Chemistry Biophysics Surface modification Mesenchymal stem cell proliferation 0210 nano-technology ComputingMilieux_MISCELLANEOUS |
| Zdroj: | European Polymer Journal European Polymer Journal, Elsevier, 2018, 107, pp.202-217. ⟨10.1016/j.eurpolymj.2018.08.011⟩ |
| ISSN: | 0014-3057 1873-1945 |
| DOI: | 10.1016/j.eurpolymj.2018.08.011⟩ |
| Popis: | Natural polymers are often used to functionalize the surface of synthetic polymer scaffolds to improve their cytocompatibility for their use as biomaterial for tissue engineering. Here we used hyaluronan (HA), a glycosaminoglycan providing essential functions in human tissues, to functionalize the surface of poly(lactic acid) (PLA) films. PLA was first aminolyzed using hexane-1,6-diamine (HDA) to bring positive charges to the scaffold surface, then allowing HA immobilization by electrostatic interactions. Since HA interact specifically with various proteins, it should be considered as a bioactive species. Thus, we investigated the influence of both aminolysis and HA immobilization conditions on HA immobilization and on mesenchymal stem cells (MSC) proliferation. Results show that (i) there is an optimum in the number of HDA molecules grafted per surface unit to reach the highest surface concentration of hydrated HA, (ii) MSC proliferation is maximal for a low surface concentration of hydrated HA (20–100 ng·cm−2), and (iii) whatever the surface concentration of hydrated HA, the MSC proliferation level depends on aminolysis conditions. We conclude that conditions used for PLA aminolysis and HA immobilization influence MSC proliferation by affecting the organization of HA molecules on scaffold surface and consequently their ability to interact with MSC membrane receptors. |
| Databáze: | OpenAIRE |
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