Analysis of the Gene Expression and RNAi-Mediated Knockdown of Chitin Synthase from Leaf-Cutting Ant Atta sexdens
| Autor: | Mariana F. Fracola, Renato Lajarim Carneiro, Odair Correa Bueno, Ariele C. Moreira, Kelli Cristina Micocci, Dulce Helena Ferreira de Souza |
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| Přispěvatelé: | Universidade Federal de São Carlos (UFSCar), Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Gene knockdown
CHS gene expression RNAi-mediated knockdown media_common.quotation_subject leaf-cutting ant fungi RNA General Chemistry Insect Chitin synthase Biology biology.organism_classification Cell biology Atta sexdens RNA interference Gene expression biology.protein CHS insect topology Gene media_common chitin synthase (CHS) |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Journal of the Brazilian Chemical Society, Volume: 31, Issue: 10, Pages: 1979-1990, Published: 09 OCT 2020 Journal of the Brazilian Chemical Society v.31 n.10 2020 Journal of the Brazilian Chemical Society Sociedade Brasileira de Química (SBQ) instacron:SBQ |
| ISSN: | 0103-5053 |
| Popis: | Made available in DSpace on 2021-06-25T12:21:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01. Added 1 bitstream(s) on 2021-07-15T15:06:02Z : No. of bitstreams: 1 S0103-50532020001001979.pdf: 5479103 bytes, checksum: fd9e849507677a0629b1dc4309a5aa1a (MD5) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Chitin synthase (CHS) is the enzyme specifically associated with chitin synthesis, an important component of diverse organisms including insects. Two alternative spliced transcripts of the CHS gene (AsCHS-A1 and AsCHS-A2) were identified by quantitative reverse-transcription polymerase chain reaction (RT-qPCR) during the development of the leaf-cutting ant Ana sermons. Expression profiles of AsCHS-A transcripts increased from larva to pupae and decay in workers. Phylogenetic analysis showed both transcripts are classified within class A insect CASs. AsCI IS-A1 showed the highest expression level in larvae and pupae, while AsCHS-A2 is the main CHS transcript in workers. Our results suggest that these variants should be under regulation of different promoters. AsCHS-A1 has topology expected for insect CHSs, while the predicted AsCHS-A2 topology. with a missing A domain, is similar to some fungal CHSs. CHS-B (class B) was not identified in A. sexdens transcriptome. Ribonucleic acid interference (RNAi)-mediated gene silencing in pupae revealed that low reduction in CHS transcript levels (18%) was enough to cause morphological changes in the pupa exoskeleton impairing the process of cuticle sclerotization. To our knowledge, this work was the first to use and to show the feasibility of using RNA interference techniques on leaf-cutting ants. Univ Fed Sao Carlos, Dept Quim, BR-13565905 Sao Carlos, SP, Brazil Univ Fed Sao Carlos, Dept Fis Quim & Matemat, BR-18052780 Sorocaba, SP, Brazil Univ Estadual Paulista, Ctr Estudos Insetos Sociais, BR-13506900 Rio Claro, SP, Brazil Univ Estadual Paulista, Ctr Estudos Insetos Sociais, BR-13506900 Rio Claro, SP, Brazil CNPq: 160662/2013-3 FAPESP: 2014/12169-2 FAPESP: 2012/25299-6 FAPESP: 2017/06198-8 CAPES: 001 |
| Databáze: | OpenAIRE |
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