Bioconjugation of l -3,4-Dihydroxyphenylalanine Containing Protein with a Polysaccharide
| Autor: | Nadarajan Saravanan Prabhu, Eunjung Song, Seog K. Kim, Nataraj Chitrapriya, Nediljko Budisa, Nahum Lee, Byung-Gee Kim, Yoon Jung Jang, Sun-Gu Lee, Nagasundarapandian Soundrarajan, Hyungdon Yun, Kanagavel Deepankumar, Niraikulam Ayyadurai, Hyung Joon Cha |
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| Rok vydání: | 2011 |
| Předmět: |
Models
Molecular Pharmacology chemistry.chemical_classification Bioconjugation Molecular Structure Chemistry Organic Chemistry Biomedical Engineering Proteins Pharmaceutical Science Periodate Bioengineering Conjugated system Combinatorial chemistry Amino acid Quinone Levodopa chemistry.chemical_compound Residue (chemistry) Polysaccharides Covalent bond Organic chemistry Biotechnology Conjugate |
| Zdroj: | Bioconjugate Chemistry. 22:551-555 |
| ISSN: | 1520-4812 1043-1802 |
| DOI: | 10.1021/bc2000066 |
| Popis: | We describe the simple bioconjugation strategy in combination of periodate chemistry and unnatural amino acid incorporation. The residue specific incorporation of 3,4-dihydroxy-l-phenylalanine can alter the properties of protein to conjugate into the polymers. The homogeneously modified protein will yield quinone residues that are covalently conjugated to nucleophilic groups of the amino polysaccharide. This novel approach holds great promise for widespread use to prepare protein conjugates and synthetic biology applications. |
| Databáze: | OpenAIRE |
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