Phosphate additives determination in meat products by 31-phosphorus nuclear magnetic resonance using new internal reference standard: hexamethylphosphoroamide
| Autor: | Edward Szłyk, Paweł Hrynczyszyn |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Chromatography
Magnetic Resonance Spectroscopy Time Factors Polyphosphate Coefficient of variation Phosphorus Hydrolysis Extraction (chemistry) Analytical chemistry chemistry.chemical_element Hydrogen-Ion Concentration Reference Standards Phosphate Hempa Analytical Chemistry Phosphates Boric acid Meat Products chemistry.chemical_compound Certified reference materials chemistry Phosphorus-31 NMR spectroscopy Food Additives Food Analysis |
| Zdroj: | Talanta. 84(1) |
| ISSN: | 1873-3573 |
| Popis: | New 31P NMR internal reference standard – hexamethylphosphoroamide (HMPA) was applied for determination of added polyphosphates and their ionic forms in raw pork meat and meat products. Phosphate species were determined after extraction with a boric acid buffer (pH = 9) and EDTA solution, using internal standard (HMPA) procedure. Hexamethylphosophoroamide was also used as the NMR reference standard. Linear correlations between phosphates and polyphosphate concentrations and 31P NMR signal areas were found in the range 81–5236 mg P/dm3, presenting 95–99% recovery and variation coefficient (CV) ≤ 5%. Studied HMPA procedure revealed shorter analysis time and the same recovery (>95%) and precision (CV = 1.3–2.7%) in comparison to MDPA method. Results of phosphate determination by both 31P NMR methods were tested against the molybdenumvanadate yellow spectrophotometric method (standard PN-ISO 13730, 1999) using standard reference material (certified phosphate solution). |
| Databáze: | OpenAIRE |
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