Growth inhibition of lung cancer cells by adenosine 5'-triphosphate
| Autor: | Gardi J. Minderman-Voortman, J. H. Paul Wilson, M. Helena C. van Rooijen, Hendrik J. Agteresch, Pieter C. Dagnelie, J. Willem O. van den Berg |
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| Přispěvatelé: | Epidemiologie, RS: NUTRIM School of Nutrition and Translational Research in Metabolism |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Pathology
medicine.medical_specialty Large cell Biology medicine.disease Small-cell carcinoma Molecular biology respiratory tract diseases chemistry.chemical_compound Papillary adenocarcinoma Epidermoid carcinoma chemistry Cell culture Drug Discovery medicine Adenocarcinoma Growth inhibition Lung cancer |
| Zdroj: | Drug Development Research, 60, 196-203. John Wiley & Sons Inc. |
| ISSN: | 1098-2299 0272-4391 |
| Popis: | Preliminary clinical data suggest that adenosine 5'-triphosphate (ATP) may inhibit lung tumor growth. Because studies of ATP on lung cancer cells are lacking, the aim of the present study was to explore effects of extracellular ATP on the growth and morphology of human lung tumor cells. Five human lung tumor cell lines derived from tumors with different cellular characteristics, i.e., a small cell carcinoma (GLC4), a large cell carcinoma (H460), a squamous cell carcinoma (H520), a mesothelioma (MERO82), and a papillary adenocarcinoma (H441), were exposed to 0, 0.5, 1, 2, and 3 mM ATP. Total cell numbers and dead or damaged cells were measured on days 1, 2, and 3. ATP induced a significant, dose-dependent growth inhibition in GLC4, H460, H520, and MERO82 cells. In contrast, H441 cells showed already maximal inhibition at 0.5 mM. Compared to untreated control cell lines, a significant growth inhibition (mean 7 SEM) of 65 7 5% (GLC4), 59 7 5% (H460), 45 7 5% (H520), 3872% (MERO82), and 55 7 8% (H441) was shown after 3 days incubation with 3 mM ATP. ATP also induced changes in morphology and attachment to the substratum. Although not demonstrated by the Trypan Blue exclusion test, on photographs it seems that ATP induces death of GLC4 and H460 cells at higher concentrations. In conclusion, in four out of five explored lung tumor cell lines, ATP induces a dose-dependent growth inhibition. Lung adenocarcinoma cells show already maximal inhibition at the lowest tested ATP dose. There is a relationship between growth inhibition and morphology changes. Drug Dev. Res. 60:196-203 |
| Databáze: | OpenAIRE |
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