Upregulation of COX-1 and COX-2 in nasal polyps in cystic fibrosis
| Autor: | Silvia Gartner, Nicolás Cobos, Laura Pujols, César Picado, Joaquim Mullol, Antonio Moreno, Jordi Roca-Ferrer, Felix Pumarola |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Pancreatic disease Adolescent Cystic Fibrosis Blotting Western Mucous membrane of nose Cystic fibrosis Nasal Polyps Downregulation and upregulation Western blot otorhinolaryngologic diseases medicine Humans Nasal polyps RNA Messenger Nose medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction business.industry Respiratory disease Middle Aged medicine.disease Up-Regulation Editorial medicine.anatomical_structure Cyclooxygenase 2 Cyclooxygenase 1 Female business |
| Zdroj: | Thorax. 61:592-596 |
| ISSN: | 0040-6376 |
| DOI: | 10.1136/thx.2004.039842 |
| Popis: | Background: Since abnormalities in prostanoid metabolism occur in the lower airway of patients with cystic fibrosis (CF), it is likely that they could also be detected in the nose. Methods: The degree of mRNA and protein expression of cyclo-oxygenase (COX) enzymes 1 (COX-1) and 2 (COX-2) was examined using quantitative reverse competitive polymerase chain reaction (RT-PCR) and Western blot analysis in the nasal polyps from 10 patients with CF, nasal polyps from 10 non-CF patients and 11 nasal mucosa specimens. The results are presented as 10 6 cDNA molecules/μg total RNA and the densitometric ratio between protein and β-actin. Results: COX-1 mRNA levels were significantly higher in CF nasal polyps (median 2.34, 25–75th percentiles 1.6–3.2) than in the nasal mucosa (0.78, 0.11–1.21), while there was no difference with non-CF nasal polyps (1.11, 0.80–3.15). COX-1 protein levels were significantly higher in CF nasal polyps (3.63, 2.71–4.27) than in nasal mucosa (1.55, 0.66–2.33) and non-CF nasal polyps (2.19, 1.72–3.68). COX-2 mRNA was significantly higher in CF nasal polyps (3.34, 2.42–7.05) than in nasal mucosa (1.69, 0.19–3.50). No differences were found in COX-2 mRNA expression between CF and non-CF polyps (1.38, 0.12–6.07). COX-2 protein levels were also significantly higher in CF nasal polyps (0.23, 0.04–0.34) than in non-CF nasal polyps (0.011, 0.009–0.016) or nasal mucosa (0.014, 0.014–0.016). Conclusions: Upregulation in the expression of COX-1 and COX-2 could explain the high production of prostanoids reported in CF. These findings raise questions regarding the potential use of selective or non-selective COX-2 non-steroidal anti-inflammatory treatment in CF. |
| Databáze: | OpenAIRE |
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