Development of a Promising Antitumor Compound Based on Rhodium(II) Succinate Associated with Iron Oxide Nanoparticles Coated with Lauric Acid/Albumin Hybrid: Synthesis, Colloidal Stability and Cytotoxic Effect in Breast Carcinoma Cells
| Autor: | Aparecido Ribeiro de Souza, Joseilma Luciana Neves Siqueira, Matheus Oliveira da Silva, Sônia Nair Báo, Marcella Lemos Brettas Carneiro |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
Succinic Acid Biomedical Engineering Serum albumin Metal Nanoparticles Breast Neoplasms Bioengineering 02 engineering and technology 010402 general chemistry Ferric Compounds 01 natural sciences chemistry.chemical_compound Tumor Cells Cultured Zeta potential Humans Rhodium General Materials Science Bovine serum albumin Aqueous solution biology Albumin Lauric Acids Serum Albumin Bovine Succinates General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Lauric acid 0104 chemical sciences chemistry Succinic acid biology.protein Nanoparticles 0210 nano-technology Iron oxide nanoparticles Nuclear chemistry |
| Zdroj: | Journal of Nanoscience and Nanotechnology. 18:3832-3843 |
| ISSN: | 1533-4880 |
| Popis: | In this study, we report the synthesis and characterization of a new rhodium(II) succinate complex (Rh2(suc)4) and its immobilization on lauric acid bilayer-coated maghemite nanoparticles (MGH-2L/Rh2(suc)4) and subsequent adsorption with bovine serum albumin (MGH-2L/Rh2(suc)4/BSA). Rh2(suc)4 has been characterized by elemental analysis, potentiometric titration, TGA, MS, FTIR and UV-Vis analysis. The maghemite phase was confirmed by XRD, and a diameter of 10 nm was obtained by Sherrer equation. The VSM experiment showed superparamagnetic properties. TEM showed nanoparticles with a spherical shape and a mean diameter of 8.5±0.4 and 9.1 ± 0.4 nm for MGH-2L/Rh2(suc)4 and MGH-2L/Rh2(suc)4/BSA, respectively. FTIR and TGA confirmed the immobilization of Rh2(suc)4 and bovine serum albumin adsorption on superparamagnetic iron oxide. Hydrodynamic size (DH) and zeta potential (ζ) measurements were made in aqueous, NaCl and DMEM media. DH for dispersions was lower in aqueous medium, but increased in saline and DMEM media. In aqueous and saline media, ζ was not altered for MGH-2L and MGH-2L/Rh2(suc)4, but was significantly lower for MGH-2L/Rh2(suc)4/BSA. Therefore, MGH-2L/Rh2(suc)4/BSA was the most stable dispersion, meaning that BSA coating prevents aggregation more than lauric acid bilayer coating. MGH-2L/Rh2(suc)4 and MGH-2L/Rh2(suc)4/BSA dispersions induced cytotoxicity in breast carcinoma (MCF-7) and fibroblast cells in culture, and this effect was higher than that exerted by free Rh2(suc)4 and more specific to breast carcinoma cells than to fibroblasts. Therefore, we suggest that these dispersions have an important potential for future clinical applications and, thus, they should be considered a platform to enhance Rh2(suc)4 cytotoxicity, specifically in breast carcinoma. |
| Databáze: | OpenAIRE |
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