Microfluidic chip-photothermal lens microscopy for DNA hybridization assay using gold nanoparticles
| Autor: | Atefeh Abbasi-Ahd, Behnaz Abbasgholi Nejad Asbaghi, Nader Shokoufi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Metal Nanoparticles
02 engineering and technology Biosensing Techniques 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound Limit of Detection Lab-On-A-Chip Devices Microscopy Gel electrophoresis Detection limit Chromatography DNA–DNA hybridization 010401 analytical chemistry Nucleic Acid Hybridization DNA Equipment Design Photothermal therapy 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences chemistry Colloidal gold Gold 0210 nano-technology |
| Zdroj: | Analytical and bioanalytical chemistry. 411(23) |
| ISSN: | 1618-2650 |
| Popis: | A new approach employing a microchip in combination with photothermal lens microscopy has been described for a DNA hybridization assay using gold nanoparticles. The difference in adsorption propensities of single- and double-stranded DNAs on gold nanoparticles was used for a highly sensitive DNA hybridization assay through a photothermal lens effect in a femtoliter scale of detection volume. Under the optimized conditions, the results showed that the variation of photothermal lens signal in the focal volume of 105 fL (10−15 L) was linearly proportional to the target DNA concentration over the range of 50–500 nM with detection limits of 30.7 nM and 27.3 nM for target DNA I and II, respectively. The lowest amount of target DNA that was measured using gold nanoparticles was 2.6 zepto mole. The assay was completed within 5 min and the relative standard deviations (n = 8) for both target DNAs were about 2.34%. The hybridization process was proved by two different common methods including gel electrophoresis and in situ fluorescence monitoring of DNA hybridization. The performance of this detection method was investigated in diluted human serum sample as a complex sample. The recovery values were between 98 and 104.9%. |
| Databáze: | OpenAIRE |
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