Single-step detection of norovirus tuning localized surface plasmon resonance-induced optical signal between gold nanoparticles and quantum dots
| Autor: | Ankan Dutta Chowdhury, Oluwasesan Adegoke, Kenshin Takemura, Tetsuro Suzuki, Jaewook Lee, Fuyuki Abe, Vipin Kumar Deo, Enoch Y. Park, Fahmida Nasrin |
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| Rok vydání: | 2018 |
| Předmět: |
Gold nanoparticle
Materials science Biomedical Engineering Biophysics Metal Nanoparticles Localized surface plasmon 02 engineering and technology 010402 general chemistry Photochemistry 01 natural sciences Feces Limit of Detection Cadmium Compounds Electrochemistry Humans Surface plasmon resonance Selenium Compounds Caliciviridae Infections CdSeTeS Detection limit Nanocomposite Quantum dots Norovirus technology industry and agriculture General Medicine Surface Plasmon Resonance equipment and supplies 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences resonance Linear range Colloidal gold Quantum dot Gold Tellurium 0210 nano-technology Selectivity Biosensor Norovirus detection Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 122:16-24 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2018.09.024 |
| Popis: | A new method of label free sensing approach with superior selectivity and sensitivity towards virlabel-freeon is presented here, employing the localized surface plasmon resonance (LSPR) behavior of gold nanoparticles (AuNPs) and fluorescent CdSeTeS quantum dots (QDs). Inorganic quaternary alloyed CdSeTeS QDs were capped with L -cysteine via a ligand exchange reaction. Alternatively, citrate stabilized AuNPs were functionalized with 11-mercaptoundecanoic acid to generate carboxylic group on the gold surface. The carboxylic group on the AuNPs was subjected to bind covalently with the amine group of L -cysteine capped CdSeTeS QDs to form CdSeTeS QDs/AuNPs nanocomposites. The fluorescence of CdSeTeS QDs/AuNPs nanocomposite shows quenched spectrum of CdSeTeS QDs at 640 nm due to the close interaction with AuNPs. However, after successive addition of norovirus-like particles (NoV-LPs), steric hindrance-induced LSPR signal from the adjacent AuNPs triggered the fluorescence enhancement of QDs in proportion to the concentration of the target NoV-LPs. A linear range of 10−14 to 10−9 g mL−1 NoV-LPs with a detection limit of 12.1 × 10−15 g mL−1 was obtained. This method was further applied on clinically isolated norovirus detection, in the range of 102–105 copies mL−1 with a detection limit of 95.0 copies mL−1, which is 100-fold higher than commercial ELISA kit. The superiority of the proposed sensor over other conventional sensors is found in its ultrasensitive detectability at low virus concentration even in clinically isolated samples. This proposed detection method can pave an avenue for the development of high performance and robust sensing probes for detection of virus in biomedical applications. |
| Databáze: | OpenAIRE |
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