miR‐124‐3p availability is antagonized by LncRNA‐MALAT1 for Slug‐induced tumor metastasis in hepatocellular carcinoma
Autor: | Xiaoguang Yu, Zheng-Yuan Jiang, Wei Wang, Chi Liu, Xiulan Zheng, Rongjun Cui, Yujia Pan, Jialin Fan, Jiebing Tang, Jia-Hui Wan, Yu-Cui Lin |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Male Cancer Research Metastasis Mice miR‐124‐3p 0302 clinical medicine Neoplasm Metastasis Original Research Cancer Biology MALAT1 biology Liver Neoplasms Cell migration hepatocellular carcinoma Middle Aged Prognosis lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Immunohistochemistry Gene Expression Regulation Neoplastic Oncology 030220 oncology & carcinogenesis Gene Knockdown Techniques Adenocarcinoma Female RNA Interference RNA Long Noncoding Adult metastasis‐associated lung adenocarcinoma transcript 1 Carcinoma Hepatocellular Slug lcsh:RC254-282 03 medical and health sciences Cell Line Tumor medicine Animals Humans Radiology Nuclear Medicine and imaging Aged Neoplasm Staging tumor metastasis Oncogene fungi biology.organism_classification medicine.disease Xenograft Model Antitumor Assays digestive system diseases SNAI2 Disease Models Animal MicroRNAs 030104 developmental biology ROC Curve Tumor progression Cancer research Snail Family Transcription Factors |
Zdroj: | Cancer Medicine, Vol 8, Iss 14, Pp 6358-6369 (2019) Cancer Medicine |
ISSN: | 2045-7634 |
Popis: | Background As an oncogene, long noncoding RNA metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1) can promote tumor metastasis. Hyperexpression of MALAT1 has been observed in many malignant tumors, including hepatocellular carcinoma (HCC). However, the role and mechanism of MALAT1 in HCC remain unclear. Methods Thirty human HCC and paracancerous tissue specimens were collected, and the human hepatoma cell lines Huh7 and HepG2 were cultured according to standard methods. MALAT1 and Snail family zinc finger (Slug) expression were measured by real‐time PCR, immunohistochemistry, and western blotting. Luciferase reporter assay and RNA immunoprecipitation (RIP) assay verified the direct interaction between miR‐124‐3p and Slug(SNAI2) or MALAT1. Wound healing and transwell assays were performed to examine invasion and migration, and a subcutaneous tumor model was established to measure tumor progression in vivo. Results MALAT1 expression was upregulated in HCC tissues and positively correlated with Slug expression. MALAT1 and miR‐124‐3p bind directly and reversibly to each other. MALAT1 silencing inhibited cell migration and invasion. miR‐124‐3p inhibited HCC metastasis by targeting Slug. Conclusions MALAT1 regulates Slug through miR‐124‐3p, affecting HCC cell metastasis. Thus, the MALAT1/miR‐124‐3p/Slug axis plays an important role in HCC. Metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1) regulates Slug through miR‐124‐3p, affecting cell migration and cell invasion. Thus, the MALAT1/miR‐124‐3p/Slug axis plays an important role in hepatocellular carcinoma. |
Databáze: | OpenAIRE |
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