miR‐124‐3p availability is antagonized by LncRNA‐MALAT1 for Slug‐induced tumor metastasis in hepatocellular carcinoma

Autor: Xiaoguang Yu, Zheng-Yuan Jiang, Wei Wang, Chi Liu, Xiulan Zheng, Rongjun Cui, Yujia Pan, Jialin Fan, Jiebing Tang, Jia-Hui Wan, Yu-Cui Lin
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
Cancer Research
Metastasis
Mice
miR‐124‐3p
0302 clinical medicine
Neoplasm Metastasis
Original Research
Cancer Biology
MALAT1
biology
Liver Neoplasms
Cell migration
hepatocellular carcinoma
Middle Aged
Prognosis
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Immunohistochemistry
Gene Expression Regulation
Neoplastic
Oncology
030220 oncology & carcinogenesis
Gene Knockdown Techniques
Adenocarcinoma
Female
RNA Interference
RNA
Long Noncoding
Adult
metastasis‐associated lung adenocarcinoma transcript 1
Carcinoma
Hepatocellular
Slug
lcsh:RC254-282
03 medical and health sciences
Cell Line
Tumor
medicine
Animals
Humans
Radiology
Nuclear Medicine and imaging
Aged
Neoplasm Staging
tumor metastasis
Oncogene
fungi
biology.organism_classification
medicine.disease
Xenograft Model Antitumor Assays
digestive system diseases
SNAI2
Disease Models
Animal
MicroRNAs
030104 developmental biology
ROC Curve
Tumor progression
Cancer research
Snail Family Transcription Factors
Zdroj: Cancer Medicine, Vol 8, Iss 14, Pp 6358-6369 (2019)
Cancer Medicine
ISSN: 2045-7634
Popis: Background As an oncogene, long noncoding RNA metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1) can promote tumor metastasis. Hyperexpression of MALAT1 has been observed in many malignant tumors, including hepatocellular carcinoma (HCC). However, the role and mechanism of MALAT1 in HCC remain unclear. Methods Thirty human HCC and paracancerous tissue specimens were collected, and the human hepatoma cell lines Huh7 and HepG2 were cultured according to standard methods. MALAT1 and Snail family zinc finger (Slug) expression were measured by real‐time PCR, immunohistochemistry, and western blotting. Luciferase reporter assay and RNA immunoprecipitation (RIP) assay verified the direct interaction between miR‐124‐3p and Slug(SNAI2) or MALAT1. Wound healing and transwell assays were performed to examine invasion and migration, and a subcutaneous tumor model was established to measure tumor progression in vivo. Results MALAT1 expression was upregulated in HCC tissues and positively correlated with Slug expression. MALAT1 and miR‐124‐3p bind directly and reversibly to each other. MALAT1 silencing inhibited cell migration and invasion. miR‐124‐3p inhibited HCC metastasis by targeting Slug. Conclusions MALAT1 regulates Slug through miR‐124‐3p, affecting HCC cell metastasis. Thus, the MALAT1/miR‐124‐3p/Slug axis plays an important role in HCC.
Metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1) regulates Slug through miR‐124‐3p, affecting cell migration and cell invasion. Thus, the MALAT1/miR‐124‐3p/Slug axis plays an important role in hepatocellular carcinoma.
Databáze: OpenAIRE
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