Role of antibody to leukocytosis-promoting factor hemagglutinin and to filamentous hemagglutinin in immunity to pertussis
| Autor: | J L Cowell, C R Manclark, H Sato, K Izumiya, Y Sato |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Bordetella pertussis
Immunology Filamentous haemagglutinin adhesin Biology Pertussis toxin Microbiology Mice medicine Animals Virulence Factors Bordetella Pertussis Vaccine Antigens Bacterial Lymphokines Immunization Passive Gamma globulin Hemagglutinin biology.organism_classification Antibodies Bacterial Virology Bordetella Disease Models Animal Hemagglutinins Infectious Diseases Pertussis Toxin Interleukin-2 Pertussis vaccine Parasitology Rabbits Pertactin Research Article medicine.drug |
| Zdroj: | Infection and Immunity. 31:1223-1231 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.31.3.1223-1231.1981 |
| Popis: | Gamma globulins were obtained from rabbits hyperimmunized with the filamentous hemagglutinin (FHA) or the leukocytosis-promoting factor hemagglutinin (LPF.HA) of Bordetella pertussis. Double immunodiffusion analyses showed the antibodies to be highly specific for the homologous antigen. Intraperitoneal injection of anti-FHA or anti-LPF.HA into 10-day-old ICR mice 30 min before aerosol challenge with B. pertussis protected the mice from disease as evidenced by survival and a normal rate of gain in body weight. Both anti-LPF.HA and anti-FHA enhanced the clearance of B. pertussis from the lung and prevented leukocytosis which normally occurs after aerosol infection of mice with B. pertussis. Anti-LPF.HA, but not anti-FHA, neutralized the leukocytosis-promoting and histamine-sensitizing activities of LPF.HA when incubated with the purified protein in vitro. Anti-FHA, but not anti-LPF.HA, prevented the attachment of B. pertussis to mammalian cells (HeLa and Vero) in culture. |
| Databáze: | OpenAIRE |
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