Extraction and purification methods in downstream processing of plant-based recombinant proteins
| Autor: | Tomasz Sakowicz, Ewelina Łojewska, Tomasz Kowalczyk, Szymon Adam Olejniczak |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Alternative methods Chromatography Downstream processing Plant based Chemical Fractionation Plants Genetically Modified Bioinformatics 01 natural sciences Recombinant Proteins law.invention 03 medical and health sciences 030104 developmental biology Downstream (manufacturing) law 010608 biotechnology Recombinant DNA Biochemical engineering Purification methods Plant system Plant Proteins Biotechnology |
| Zdroj: | Protein Expression and Purification. 120:110-117 |
| ISSN: | 1046-5928 |
| DOI: | 10.1016/j.pep.2015.12.018 |
| Popis: | During the last two decades, the production of recombinant proteins in plant systems has been receiving increased attention. Currently, proteins are considered as the most important biopharmaceuticals. However, high costs and problems with scaling up the purification and isolation processes make the production of plant-based recombinant proteins a challenging task. This paper presents a summary of the information regarding the downstream processing in plant systems and provides a comprehensible overview of its key steps, such as extraction and purification. To highlight the recent progress, mainly new developments in the downstream technology have been chosen. Furthermore, besides most popular techniques, alternative methods have been described. |
| Databáze: | OpenAIRE |
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