Cellulose synthesis in Phytophthora infestans is required for normal appressorium formation and successful infection of potato
| Autor: | Anna O. Avrova, Alison Williams, Paul R. J. Birch, Laura J. Grenville-Briggs, Stephan Wawra, Johanna Fugelstad, Vincent Bulone, Victoria L. Anderson, Stephen C. Whisson, Pieter van West, Jamel Bouzenzana |
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| Rok vydání: | 2008 |
| Předmět: |
Phytophthora
Molecular Sequence Data Plant Science Microbiology Cell wall chemistry.chemical_compound Microscopy Electron Transmission RNA interference Cell Wall Gene family Electrophoresis Gel Two-Dimensional Amino Acid Sequence Cellulose Phylogeny Research Articles Plant Diseases Solanum tuberosum Oomycete Appressorium biology Models Genetic Sequence Homology Amino Acid Reverse Transcriptase Polymerase Chain Reaction Algal Proteins food and beverages Cell Biology biology.organism_classification chemistry Glucosyltransferases Phytophthora infestans RNA Interference |
| Zdroj: | The Plant cell. 20(3) |
| ISSN: | 1040-4651 |
| Popis: | Cellulose, the important structural compound of cell walls, provides strength and rigidity to cells of numerous organisms. Here, we functionally characterize four cellulose synthase genes (CesA) in the oomycete plant pathogen Phytophthora infestans, the causal agent of potato (Solanum tuberosum) late blight. Three members of this new protein family contain Pleckstrin homology domains and form a distinct phylogenetic group most closely related to the cellulose synthases of cyanobacteria. Expression of all four genes is coordinately upregulated during pre- and early infection stages of potato. Inhibition of cellulose synthesis by 2,6-dichlorobenzonitrile leads to a dramatic reduction in the number of normal germ tubes with appressoria, severe disruption of the cell wall in the preinfection structures, and a complete loss of pathogenicity. Silencing of the entire gene family in P. infestans with RNA interference leads to a similar disruption of the cell wall surrounding appressoria and an inability to form typical functional appressoria. In addition, the cellulose content of the cell walls of the silenced lines is >50% lower than in the walls of the nonsilenced lines. Our data demonstrate that the isolated genes are involved in cellulose biosynthesis and that cellulose synthesis is essential for infection by P. infestans. |
| Databáze: | OpenAIRE |
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