Introduction and Expression of the cry1Ac Gene of Bacillus thuringiensis in a Cereal-Associated Bacterium, Bacillus polymyxa
| Autor: | Vaithilingam Sekar, S.N. Sudha, R. Jayakumar |
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| Rok vydání: | 1999 |
| Předmět: |
Crops
Agricultural Scirpophaga incertulas Bacterial Toxins Immunoblotting Bacillus thuringiensis Gene Expression Bacillus Transfection Applied Microbiology and Biotechnology Microbiology Hemolysin Proteins Bacterial Proteins Botany Animals Oryza sativa Bacillus thuringiensis Toxins biology fungi food and beverages Oryza General Medicine biology.organism_classification Recombinant Proteins Endotoxins Biopesticide Cry1Ac Genes Bacterial Paenibacillus polymyxa Phyllosphere |
| Zdroj: | Current Microbiology. 38:163-167 |
| ISSN: | 0343-8651 |
| DOI: | 10.1007/pl00006781 |
| Popis: | The abilities of Bacillus polymyxa and Bacillus thuringiensis to survive on the rice phyllospere were compared; it was found that B. polymyxa colonizes the crop better. This study also showed that B. polymyxa inoculation to rice plants increased the shoot and the root growth of the crop. Efforts were made to introduce the cry1Ac gene of B. thuringiensis subsp. kurstaki into B. polymyxa so that the application of such transgenic B. polymyxa strains would prove to be dually beneficial to rice crops both as a biopesticide and as a biofertilizer. Immunoblot analysis of the recombinant organism containing the cry1Ac gene, strain BP113, indicated efficient expression of this gene in the heterologous host. Bioassays with the first instar larvae of the yellow stem borer of rice (Scirpophaga incertulas) revealed that the protein preparations from BP113 were toxic. |
| Databáze: | OpenAIRE |
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