Adenovirus ELISA for the evaluation of cerebrospinal fluid in patients with suspected neurosyphilis
| Autor: | Erich H. Cerny, Sandra A. Larsen, Francis K. Lee, E A Hambie, Carol E. Farshy |
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| Rok vydání: | 1985 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Central nervous system Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Fluorescent treponemal antibody absorption test Antibodies Viral Adenoviridae Neurosyphilis Cerebrospinal fluid Antigen medicine Humans Treponema medicine.diagnostic_test biology business.industry Antibody titer General Medicine Middle Aged medicine.disease medicine.anatomical_structure Immunology biology.protein Syphilis Female Antibody business |
| Zdroj: | American journal of clinical pathology. 84(4) |
| ISSN: | 0002-9173 |
| Popis: | An enzyme-linked immunosorbent assay (ELISA) detecting antibodies against the adenovirus group antigen was used to examine cerebrospinal fluid (CSF) of suspected neurosyphilis patients for serum contamination or blood–brain barrier (BBB) leakage. Adenovirus antibodies are ubiquitous, produce high antibody titers, and rarely cause central nervous system (CNS) infections. Of 52 normal adult sera tested with this ELISA, only one lacked antibodies. CSF from 48 healthy individuals did not present a detectable amount of ariti-adenovirus antibodies. CSF from 33 suspected neurosyphilis patients with positive results in the fluorescent treponemal antibody–absorption (FTA-ABS)–CSF test were examined. Eighteen showed anti-adenovirus antibodies indicating contamination of the CSF with peripheral blood or damaged BBB by syphilis or other disease, resulting in questionable CSF treponemal results. The remaining 15 of these patients appeared to be producing their anti-treponemal antibodies in the CNS. This procedure may prove to be of considerable help in excluding false positive FTA-ABS results in CSF samples. |
| Databáze: | OpenAIRE |
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