Nasal Polyposis Pathogenesis: A Flow Cytometric and Immunohistochemical Study of Epithelial CeU Proliferation
| Autor: | Jean-François Bernaudin, R. Peynegre, André Coste, Rateau Jg, Estelle Escudier |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Pathology
medicine.medical_specialty Biology Epithelium Flow cytometry chemistry.chemical_compound Nasal Polyps otorhinolaryngologic diseases medicine Humans Nasal polyps Propidium iodide medicine.diagnostic_test Cell growth Epithelial Cells General Medicine Hyperplasia Flow Cytometry medicine.disease Immunohistochemistry Squamous metaplasia Nasal Mucosa medicine.anatomical_structure Otorhinolaryngology chemistry Respiratory epithelium Cell Division |
| Zdroj: | Acta Oto-Laryngologica. 116:755-761 |
| ISSN: | 1651-2251 0001-6489 |
| Popis: | In nasal polyps, constantly associated with chronic inflammation, frequent epithelial morphological changes (squamous metaplasia, secretory hyperplasia) suggest a dysregulation of epithelial cell proliferation. Cell proliferation in nasal respiratory epithelium was therefore evaluated in nasal polyposis. In 20 patients, we compared cell proliferation in mucosa from the inferior turbinate to these in nasal polyps using two methods: Flow cytometry analyzing first the ploidy and the percentage of S-phase cells (propidium iodide DNA labeling), secondly the percentage of Ki-67-labeled cells and the green fluorescent index (fluorescein-conjugated anti-human Ki-67 antigen labeling, and thirdly the percentage of Ki-67-labeled cells being in S-phase. Immunohistochemistry, quantifying the expression of Ki-67 antigen in the epithelium permitting to calculate a Ki-67 index. All cell-populations studied were diploid. Percentages of S-phase cells, Ki-67-labeled cells, Ki-67 labeled cells being in the S-phase and green fluorescence index was significantly higher in nasal polyps than in mucosa Ki-67 index were significantly higher in nasal polyps than in mucosa in the epithelium. Epithelial cell proliferation which is therefore increased in nasal polyp could play an important role in nasal polyposis pathogenesis and its relationships with inflammation can be suggested. |
| Databáze: | OpenAIRE |
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