A microfluidic device for monitoring siRNA delivery under fluid flow

Autor:	van der Meer, Andries Dirk, Feijen, J., Kamphuis, Marloes, Hennink, W.E., Poot, Andreas A., Sam, A.P., Feijen, Jan, Vermes, I.
Přispěvatelé:	Faculty of Science and Technology, Biomaterials Science and Technology
Rok vydání:	2008
Předmět:	Liposome METIS-254631 Chemistry METIS-255845 Microfluidics Pharmaceutical Science Nanotechnology Transfection Fluorescence In vitro In vivo IR-69301 Fluid dynamics Biophysics Particle
Zdroj:	ISSUE=10;STARTPAGE=181;ENDPAGE=184;TITLE=10th European Symposium on Controlled Drug Delivery, ESCDD 2008 ISSUE=10;STARTPAGE=e42;ENDPAGE=e44;TITLE=10th European Symposium on Controlled Drug Delivery, ESCDD 2008
Popis:	When studying particle uptake in vitro, it is favorable to mimic the in vivo situation as much as possible. In this study we present a microfluidic device to mimic the mechanical stress caused by the flow of blood while studying particle uptake in vitro. Human endothelial cells were treated with liposomes containing fluorescent siRNA. It was found that applying physiologically relevant mechanical stress during transfection diminishes the uptake of liposomes in the cells.
Databáze:	OpenAIRE
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