Biochemical properties and crystal structure of isocitrate lyase from Bacillus cereus ATCC 14579
Autor: | Il-Kwon Kim, Kyung-Jin Kim, Sangwoo Kim, Dongwoo Ki, Seul Hoo Lee |
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Rok vydání: | 2020 |
Předmět: |
Models
Molecular 0301 basic medicine Stereochemistry Succinic Acid Biophysics Glyoxylate cycle Bacillus cereus Crystallography X-Ray Biochemistry Enzyme catalysis 03 medical and health sciences Acetic acid chemistry.chemical_compound 0302 clinical medicine Sequence Analysis Protein Catalytic Domain Magnesium Molecular Biology chemistry.chemical_classification biology Glyoxylates Active site Cell Biology Isocitrate lyase biology.organism_classification Isocitrate Lyase Enzyme assay 030104 developmental biology Enzyme chemistry Metals 030220 oncology & carcinogenesis biology.protein Protein Multimerization Sequence Alignment |
Zdroj: | Biochemical and Biophysical Research Communications. 533:1177-1183 |
ISSN: | 0006-291X |
DOI: | 10.1016/j.bbrc.2020.09.136 |
Popis: | The glyoxylate cycle is an important anabolic pathway and acts under a C2 compound (such as acetic acid) rich condition in bacteria. The isocitrate lyase (ICL) enzyme catalyzes the first step in the glyoxylate cycle, which is the cleavage of isocitrate to glyoxylate and succinate. This enzyme is a metalo-enzyme that contains an Mg2+ or a Mn2+ion at the active site for enzyme catalysis. We expressed and purified ICL from Bacillus cereus (BcICL) and investigated its biochemical properties and metal usage through its enzyme activity and stability with various divalent metal ion. Based on the results, BcICL mainly utilized the Mg2+ ion for enzyme catalysis as well as the Mn2+, Ni2+ and Co2+ ions. To elucidate its molecular mechanisms, we determined the crystal structure of BcICL at 1.79 A. Through this structure, we analyzed a tetrameric interaction of the protein. We also determined the BcICL structure in complex with both the metal and its products, glyoxylate and succinate at 2.50 A resolution and revealed each ligand binding modes. |
Databáze: | OpenAIRE |
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