Isolation and characterization of sheep lactoferrin, an inhibitor of platelet aggregation and comparison with human lactoferrin
| Autor: | Pierre Jollès, D. Migliore-Samour, Anne-Marie Fiat, Zu-Yuan Qian |
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| Rok vydání: | 1995 |
| Předmět: |
Models
Molecular Platelet aggregation Platelet Aggregation Molecular Sequence Data Biophysics Biochemistry Hydrolysate Sequence determination fluids and secretions Pepsin Species Specificity Animals Humans Platelet Amino Acid Sequence Molecular Biology Chromatography Sheep biology Sequence Homology Amino Acid Chemistry Lactoferrin food and beverages Fast protein liquid chromatography Peptide Fragments stomatognathic diseases Electrophoresis Milk biology.protein Sequence Analysis Platelet Aggregation Inhibitors Protein Binding |
| Zdroj: | Biochimica et biophysica acta. 1243(1) |
| ISSN: | 0006-3002 |
| Popis: | Highly purified sheep lactoferrin was isolated from ovine whey in a single chromatographic step (FPLC): it was characterized by electrophoresis, N-terminal sequence determination and compared with lactoferrins from other species. Sheep and human lactoferrins inhibited thrombin-induced platelet aggregation (median inhibitory concentration: IC 50 5 and 4 μM. respectively). Pepsin hydrolysates of human and sheep lactoferrins were fractionated by reverse-phase high-performance liquid chromatography and only one peak was an inhibitor of platelet aggregation. The sheep or human lactoferrin binding to platelets was studied. |
| Databáze: | OpenAIRE |
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