Altered Expression of Retinol Metabolism-Related Genes in an ANIT-Induced Cholestasis Rat Model

Autor: Akiko Inoue, Kimitaka Takitani, Kanta Kishi, Hiroshi Tamai, Hiroshi Miyazaki, Hirofumi Tamaki, Maki Koh
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
Receptors
Retinoic Acid
Retinoic acid
Receptors
Cytoplasmic and Nuclear
Aldehyde dehydrogenase
lcsh:Chemistry
chemistry.chemical_compound
Vitamin A
lcsh:QH301-705.5
Spectroscopy
Cholestasis
biology
Bile acid
Chemistry
Retinol
General Medicine
Retinoic Acid 4-Hydroxylase
Computer Science Applications
1-Naphthylisothiocyanate
retinol
medicine.medical_specialty
medicine.drug_class
digestive system
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
CYP26A1
Internal medicine
medicine
bile acid
Animals
Rats
Wistar
Physical and Theoretical Chemistry
Molecular Biology
beta-Carotene 15
15'-Monooxygenase
Organic Chemistry
Cytochrome P450
Aldehyde Dehydrogenase
medicine.disease
Rats
Disease Models
Animal
030104 developmental biology
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
Gene Expression Regulation
biology.protein
Farnesoid X receptor
farnesoid X receptor
Acyltransferases
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 19, Iss 11, p 3337 (2018)
Volume 19
Issue 11
ISSN: 1422-0067
Popis: Cholestasis is defined as a reduction of bile secretion caused by a dysfunction of bile formation. Insufficient bile secretion into the intestine undermines the formation of micelles, which may result in the reduced absorption of lipids and fat-soluble vitamins. Here, we investigated the retinol homeostasis and the alterations of retinol metabolism-related genes, including &beta
carotene 15,15&prime
monooxygenase (BCMO), lecithin:retinol acyltransferase (LRAT), aldehyde dehydrogenase (ALDH), cytochrome P450 26A1 (CYP26A1), and retinoic acid receptors (RAR) &beta
in a &alpha
naphthyl isothiocyanate (ANIT)-induced cholestasis rat model. Moreover, we examined the expression of the farnesoid X receptor (FXR) target genes. Our results showed that plasma retinol levels were decreased in ANIT rats compared to control rats. On the contrary, hepatic retinol levels were not different between the two groups. The expression of FXR target genes in the liver and intestine of cholestasis model rats was repressed. The BCMO expression was decreased in the liver and increased in the intestine of ANIT rats compared to control rats. Finally, the hepatic expression of LRAT, RAR&beta
and ALDH1A1 in cholestatic rats was decreased compared to the control rats, while the CYP26A1 expression of the liver was not altered. The increased expression of intestinal BCMO in cholestasis model rats might compensate for decreased circulatory retinol levels. The BCMO expression might be regulated in a tissue-specific manner to maintain the homeostasis of retinol.
Databáze: OpenAIRE
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