| Autor: |
Falvo, Elisabetta, Damiani, Verena, Giamaica Conti, Boschi, Federico, Messana, Katia, Giacomini, Patrizio, Milella, Michele, Laurenzi, Vincenzo De, Morea, Veronica, Sala, Gianluca, Fracasso, Giulio, Ceci, Pierpaolo |
| Rok vydání: |
2021 |
| DOI: |
10.6084/m9.figshare.13889356 |
| Popis: |
Additional file 1: Table S1. Free-drug content in lyophilized The-0504 after storage at 2–8 °C for eight months. Fig. S1. The-05 binding to pancreatic PaCa44 cells. Flow cytometric analysis of The-05. Mitoxantrone-containing The-05 (The-05-Mit) preparation was incubated for different times at 37 °C with human PaCa44 cells. Binding was revealed by direct fluorescence reading in a BD FACSCanto II flow cytometer. The-05-Mit provided at the predetermined optimal concentration of 0.5 μM (in Mitoxantrone). MFI refers to the median fluorescence intensity of the sample subtracted from the cell autofluorescence value. Fig. S2. MMP-9 and CD71 expression evaluation in cancer cells. Western blot using anti-MMP-9 antibody on SJSA-1 cells (A) or anti-CD71 antibody on SJSA-1, Panc-1 and MIA-PaCa-2 cells (B). GAPDH was used as a loading control. Fig. S3. Anti-tumor activity of The-0504 in mice bearing subcutaneous HepG2 liver tumors. Tumor-growth curves for each mouse groups are indicated. Animals were observed up to 60 days. At that time, 100% of The-0504-treated mice were still alive and with any residual sign of disease. Statistical significance according to the Student’s t-test: control vs The-0504 * p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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