Human papillomavirus detected in sperm of Japanese infertile males affects reproductive parameters
| Autor: | Masashi Iijima, Yoshifumi Kadono, Kouji Izumi, Shohei Kawaguchi, Yuki Kato, Hiroki Nakata, Kazuyoshi Shigehara, Kazufumi Nakashima, Tomomi Nakagawa, Atsushi Mizokami |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Male HPV Sperm infection Prevalence Semen Infectious and parasitic diseases RC109-216 Alphapapillomavirus Infertile men law.invention Superoxide dismutase Andrology Japan law medicine Humans Papillomaviridae Sperm motility Polymerase chain reaction biology urogenital system Papillomavirus Infections HPV infection virus diseases General Medicine medicine.disease Sperm Spermatozoa female genital diseases and pregnancy complications Sperm concentration Infectious Diseases biology.protein Reproductive parameters Nested polymerase chain reaction |
| Zdroj: | International Journal of Infectious Diseases, Vol 112, Iss, Pp 294-299 (2021) |
| ISSN: | 1201-9712 |
| Popis: | Objectives The effects of human papillomavirus (HPV) infection on male reproductive parameters are currently a matter of controversy. In order to clarify the issue in Japanese infertile men, the prevalence and localization of HPV in semen, sperm parameters, and superoxide dismutase (SOD) activity in seminal plasma were examined in 216 Japanese infertile men. Methods DNA was extracted from liquid-based cytological semen samples. The β-globin gene was amplified by polymerase chain reaction (PCR), and HPV-DNA was amplified using nested PCR with MY09/MY11 as outer primers and GP5+/GP6+ as inner primers. HPV genotyping was performed in the HPV-positive samples. In addition, SOD levels in seminal plasma were analysed quantitatively. In-situ hybridization (ISH) was performed to localize HPV-DNA in sperm from HPV-positive samples. Results Any-risk and high-risk prevalence rates of HPV in semen were 12.5% and 6.9%, respectively. No significant difference in the prevalence of HPV was observed between azoospermic and non-azoospermic subjects. Among non-azoospermic patients, those with HPV detected in semen had significantly lower sperm motility and concentration compared with subjects without HPV detected in semen. SOD levels in seminal plasma were significantly higher in HPV-positive patients compared with HPV-negative patients. ISH analysis of HPV-positive samples revealed that HPV-DNA was localized to the head and mid-piece of sperm. HPV-DNA was present in the sperm of young infertile men. Conclusion HPV infection of sperm was associated with reduced sperm motility and concentration, and resulted in an increase in seminal SOD activity. |
| Databáze: | OpenAIRE |
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