Sustained delivery of BSA/HSA from biocompatible plant cellulose nanocrystals for in vitro cholesterol release from endothelial cells
| Autor: | Yogendra Padwad, Rubbel Singla, Sourabh Soni, Amitabha Acharya, Sudesh Kumar Yadav |
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| Rok vydání: | 2017 |
| Předmět: |
Surface Properties
Nanoparticle Biocompatible Materials 02 engineering and technology Plasma protein binding 010402 general chemistry 01 natural sciences Biochemistry chemistry.chemical_compound Adsorption Structural Biology Human Umbilical Vein Endothelial Cells Animals Humans Cellulose Molecular Biology Drug Carriers Nanocomposite Serum Albumin Bovine General Medicine Plants 021001 nanoscience & nanotechnology In vitro 0104 chemical sciences Cholesterol chemistry Biophysics Nanoparticles Cattle 0210 nano-technology Selectivity Drug carrier |
| Zdroj: | International journal of biological macromolecules. 104 |
| ISSN: | 1879-0003 |
| Popis: | Nanocomposites of plant cellulose nanocrystals (CNCs) were developed by binding model proteins BSA and HSA onto CNCs by physical adsorption and chemical conjugation methods The spectroscopy and microscopy studies confirmed the protein binding onto CNCs. Phosphate buffer saline (pH=4.0, 7.4) and simulated gastric and intestinal fluids (SGF/SIF; pH=1.1/6.5) showed maximum protein release of ∼62% over a period of time. The released proteins were found to retain both structural integrity as well as≥90% of bioactivity. Further, these cytocompatible nanocomposites showed ∼58-85% cholesterol release from HUVEC whereas no selectivity was observed for HCAEC. It is speculated that due to the presence of combination of shuttles (albumins) and sinks (CNCs and albumins), these prepared nanocomposites with increased cholesterol effluxing ability may serve as a potential candidate for future biomedical applications in pharmaceuticals. |
| Databáze: | OpenAIRE |
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