Watching cellular machinery in action, one molecule at a time
| Autor: | Antoine M. van Oijen, Enrico Monachino, Lisanne M. Spenkelink |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
DNA Replication
0301 basic medicine Magnetic tweezers Optical Tweezers Cytological Techniques Ensemble averaging Complex system Reviews High resolution Review Biosensing Techniques Computational biology Biology Microscopy Atomic Force Models Biological Magnetics 03 medical and health sciences Molecular dynamics Fluorescence Resonance Energy Transfer Animals Humans LIVING CELLS SINGLE-MOLECULE IN-VIVO ATOMIC-FORCE MICROSCOPY ELECTRON-MICROSCOPY Atomic force microscopy Molecular Motor Proteins DNA-REPLICATION technology industry and agriculture DNA replication Cell Biology MAGNETIC TWEEZERS Molecular Imaging Cytoskeletal Proteins 030104 developmental biology Microscopy Fluorescence Action (philosophy) BIOMOLECULAR PROCESSES ESCHERICHIA-COLI Biophysics HIGH-RESOLUTION |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Monachino et al. review recent developments in single-molecule biophysical approaches and the cell biological advances they allow. Single-molecule manipulation and imaging techniques have become important elements of the biologist’s toolkit to gain mechanistic insights into cellular processes. By removing ensemble averaging, single-molecule methods provide unique access to the dynamic behavior of biomolecules. Recently, the use of these approaches has expanded to the study of complex multiprotein systems and has enabled detailed characterization of the behavior of individual molecules inside living cells. In this review, we provide an overview of the various force- and fluorescence-based single-molecule methods with applications both in vitro and in vivo, highlighting these advances by describing their applications in studies on cytoskeletal motors and DNA replication. We also discuss how single-molecule approaches have increased our understanding of the dynamic behavior of complex multiprotein systems. These methods have shown that the behavior of multicomponent protein complexes is highly stochastic and less linear and deterministic than previously thought. Further development of single-molecule tools will help to elucidate the molecular dynamics of these complex systems both inside the cell and in solutions with purified components. |
| Databáze: | OpenAIRE |
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