Recurrent chromosomal abnormalities in human embryonic stem cells

Autor:	Mieke Geens, Yves Vandeskelde, Josiane Van der Elst, Catherine Staessen, Afroditi Mertzanidou, Ileana Mateizel, Claudia Spits, Inge Liebaers, Karen Sermon
Přispěvatelé:	Department of Embryology and Genetics
Předmět:	Derivative chromosome Molecular Sequence Data Biomedical Engineering Chromosomes Human Pair 20 Aneuploidy Bioengineering Biology Applied Microbiology and Biotechnology Cell Line Gene duplication medicine Humans MOLECULAR KARYOTYPING Embryonic Stem Cells In Situ Hybridization Fluorescence Chromosome Aberrations Comparative Genomic Hybridization Gene Amplification Karyotype human embryonic stem cells medicine.disease Molecular biology Embryonic stem cell Chromosome Banding Cell culture Karyotyping Molecular Medicine Stem cell Chromosomes Human Pair 18 Biotechnology Comparative genomic hybridization
Zdroj:	Vrije Universiteit Brussel
Popis:	Cultured human embryonic stem (hES) cells have a known predisposition to aneuploidy of chromosomes 12, 17 and X. We studied 17 hES cell lines by array-based comparative genomic hybridization (aCGH) and found that the cells accumulate other recurrent chromosomal abnormalities, including amplification at 20q11.21 and a derivative chromosome 18. These genomic changes have a variable impact at the transcriptional level.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22706b97842fd12e0203da4fe49c33fc https://researchportal.vub.be/en/publications/6a25b390-c2a5-4b57-b929-eaa817b78054 Zobrazit plný text záznamu