Dietary Selenium Deficiency or Excess Reduces Sperm Quality and Testicular mRNA Abundance of Nuclear Glutathione Peroxidase 4 in Rats
| Autor: | Huimin Zhang, Yuanfei Xu, Chunmei Gong, Xiongshun Liang, Xiao-Li Liu, Xin Gen Lei, Junluan Mo, Shi-jie Zheng, Ji-Chang Zhou |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Medicine (miscellaneous) chemistry.chemical_element Biology Rats Sprague-Dawley 03 medical and health sciences Selenium Selenium deficiency Internal medicine Selenoprotein P Testis medicine Weaning Animals RNA Messenger Sperm motility chemistry.chemical_classification Glutathione Peroxidase Nutrition and Dietetics Glutathione peroxidase Deoxyguanosine medicine.disease Phospholipid Hydroperoxide Glutathione Peroxidase Sperm Spermatozoa Diet Nutrition Disorders 030104 developmental biology Endocrinology chemistry Liver 8-Hydroxy-2'-Deoxyguanosine Selenoprotein Deficiency Diseases |
| Zdroj: | The Journal of nutrition. 147(10) |
| ISSN: | 1541-6100 |
| Popis: | Background: Glutathione peroxidase (GPX) 4 and selenoprotein P (SELENOP) are abundant, and several variants are expressed in the testis.Objective: We determined the effects of dietary selenium deficiency or excess on sperm quality and expressions of GPX4 and SELENOP variants in rat testis and liver.Methods: After weaning, male Sprague-Dawley rats were fed a Se-deficient basal diet (BD) for 5 wk until they were 9 wk old [mean ± SEM body weight (BW) = 256 ± 5 g]. They were then fed the BD diet alone (deficient) or with 0.25 (adequate), 3 (excess), or 5 (excess) mg Se/kg for 4 wk. Testis, liver, blood, and semen were collected to assay for selenoprotein mRNA and protein abundances, selenium concentration, GPX activity, 8-hydroxy-deoxyguanosine concentration, and sperm quality.Results: Dietary selenium supplementations elevated (P < 0.05) tissue selenium concentrations and GPX activities. Compared with those fed BD + 0.25 mg Se/kg, rats fed BD showed lower (P < 0.05) BW gain (86%) and sperm density (57%) but higher (P < 0.05) plasma 8-hydroxy-deoxyguanosine concentrations (189%), and nonprogressive sperm motility (4.4-fold). Likewise, rats fed BD + 5 mg Se/kg had (P = 0.06) lower BW gain and higher (1.9-fold) sperm deformity rates than those in the selenium-adequate group. Compared with the selenium-adequate group, dietary selenium deficiency (BD) or excess (BD + 3 or 5 mg Se/kg) resulted in 45-77% lower (P < 0.05) nuclear Gpx4 (nGpx4) mRNA abundance in the testis. Rats fed BD had lower (P < 0.05) mRNA levels of 2 Selenop variants in both testis and liver than those in the other groups. Testicular SELENOP was 155-170% higher (P < 0.05) in rats fed BD + 5 mg Se/kg and hepatic c/mGPX4 was 13-15% lower (P < 0.05) in rats fed BD than in the other groups.Conclusions: The mRNA abundance of rat testicular nGPX4 responded to dietary selenium concentrations in similar ways to sperm parameters and may be used as a sensitive marker to assess appropriate Se status for male function. |
| Databáze: | OpenAIRE |
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