Cannabidiol promotes fin regeneration and reduces apoptosis in zebrafish embryos
| Autor: | Huan Zhang, Ping-Shan Lai, Shisan Xu, Xueping Chen, Shuk Han Cheng, Cun-Zhao Li, Guijiang Wang, Yu Suen Chan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Poly ADP ribose polymerase
Medicine (miscellaneous) Caspase 3 Apoptosis digestive system Fin regeneration medicine Cannabidiol Regeneration TX341-641 Zebrafish Inflammation Nutrition and Dietetics biology Chemistry Nutrition. Foods and food supply Regeneration (biology) biology.organism_classification digestive system diseases Cell biology surgical procedures operative Toxicity Food Science medicine.drug |
| Zdroj: | Journal of Functional Foods, Vol 86, Iss, Pp 104694-(2021) |
| ISSN: | 1756-4646 |
| Popis: | Cannabidiol (CBD) extracts have gained attention for their therapeutic efficacy. The present study used a zebrafish embryo caudal fin amputation model and found 1.25 mg/L crude CBD extract accelerated caudal fin regeneration and reduced apoptosis after amputation, while not altering mitotic regenerating cells. The purity of the CBD extract was 55.5 ± 0.37%. Pure CBD promoted fin regeneration and inhibited neutrophil accumulation in a dose-dependent manner, while these effects were not seen in a high concentration of CBD (2.5 mg/L), possibly due to the toxicity of the high dose. An equivalent amount of pure CBD (0.625 to 1.25 mg/L) enhanced regeneration and reduced apoptosis similarly to crude CBD extract (1.25 mg/L). Additionally, CBD reduced the gene expression level of cxcl8, tnf-a, and il-1β, and protein expression level of IL-1β, Caspase 3, and PARP, but not Bcl-2 or Bax. These results suggest that CBD exerts its effect on wound pro-regeneration in part by modulating the inflammatory response and the IL-1β/Caspase 3/PARP signal pathway. |
| Databáze: | OpenAIRE |
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