Effect of Methanolic Leaf Extract of Ocimum basilicum L. on Benzene-Induced Hematotoxicity in Mice
| Autor: | Soumen Saha, M. K. Mukhopadhyay, Debjani Nath, Parthadeb Ghosh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
food.ingredient
Article Subject Monoterpene Pharmacology Citral law.invention Toxicology chemistry.chemical_compound food Downregulation and upregulation law Medicine Essential oil biology business.industry Basilicum lcsh:Other systems of medicine Ocimum biology.organism_classification lcsh:RZ201-999 Complementary and alternative medicine chemistry DNA fragmentation business Geraniol Research Article |
| Zdroj: | Evidence-Based Complementary and Alternative Medicine, Vol 2012 (2012) Evidence-based Complementary and Alternative Medicine : eCAM |
| ISSN: | 1741-4288 |
| Popis: | The aim of the present study was to investigate the protective role of methanolic leaf extract ofOcimum basilicumL. against benzene-induced hematotoxicity in Swiss albino mice. GC analysis and subacute toxicity level of the extract were tested. Mice were randomly divided into three groups among which II and III were exposed to benzene vapour at a dose 300 ppm × 6 hr/day × 5 days/week for 2 weeks and group I was control. Group III of this experiment was treated with the leaf methanolic extract at a dose of 100 mg/kg body weight, a dose in nontoxic range. Hematological parameters (Hb%, RBC and WBC counts), cell cycle regulatory proteins expression and DNA fragmentation analysis of bone marrow cells was performed. There was an upregulation of p53 and p21 and downregulation of levels of CDK2, CDK4, CDK6, and cyclins D1 and E in leaf extract-treated group. DNA was less fragmented in group III compared to group II (P<0.05). The present study indicates that the secondary metabolites ofO. basilicumL. methanolic leaf extract, comprising essential oil monoterpene geraniol and its oxidized form citral as major constituents, have modulatory effect in cell cycle deregulation and hematological abnormalities induced by benzene in mice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text