Galenic Lab-on-a-Chip concept for lipid nanocapsules production
| Autor: | Brice Calvignac, Jean-Christophe Gimel, Carl Simonsson, Sylvain Verron, Nicolas Rolley, Thomas Bizien, Jérémie Riou, Marie Bonnin, Laurent Robert, Guillaume Brotons, Guillaume Lefebvre, Jean-Pierre Benoit, Sylwester Bargiel |
|---|---|
| Přispěvatelé: | Université d'Angers (UA), Micro et Nanomédecines Translationnelles (MINT), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Angevin de Recherche en Ingénierie des Systèmes (LARIS), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Université Bourgogne Franche-Comté [COMUE] (UBFC), Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), Le Mans Université (UM), Institut des Molécules et Matériaux du Mans (IMMM), Le Mans Université (UM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), ANR-Euronanmed3, ANR-17-ENM3-0008,RESOLVE,SuppRESsion of immunopathology by nanOparticle deLiVEry of mRNA to monocytes(2017) |
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Microfluidics 3D printing Nanotechnology Context (language use) 02 engineering and technology 010402 general chemistry 01 natural sciences Continuous production law.invention [SPI]Engineering Sciences [physics] Nanocapsules X-Ray Diffraction law Lab-On-A-Chip Devices Scattering Small Angle Deep reactive-ion etching [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering General Materials Science business.industry Reproducibility of Results [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences Lab-on-a-chip 021001 nanoscience & nanotechnology Chip Lipids 0104 chemical sciences Characterization (materials science) 0210 nano-technology business |
| Zdroj: | Nanoscale Nanoscale, Royal Society of Chemistry, 2021, 13, pp.11899-11912. ⟨10.1039/d1nr00879j⟩ |
| ISSN: | 2040-3372 2040-3364 |
| Popis: | International audience; The continuous production of drug delivery systems assisted by microfluidics has drawn a growing interest because of the high reproducibility, low batch-to-batch variations, narrow and controlled particle size distributions and scale-up ease induced by this kind of processes. Besides, microfluidics offers opportunities for high throughput screening of process parameters and the implementation of process characterization techniques as close to the product as possible. In this context, we propose to spotlight the GALECHIP concept through the development of an instrumented microfluidic pilot considered as a Galenic Lab-on-a-Chip to formulate nanomedicines, such as lipid nanocapsules (LNCs), under controlled process conditions. In this paper we suggest an optimal rational development in terms of chip costs and designs. First, by using two common additive manufacturing techniques, namely fused deposition modelling and multi-jet modelling to prototype customized 3D microfluidic devices (chips and connectors). Secondly, by manufacturing transparent Silicon (Si)/Glass chips with similar channel geometries but obtained by a new approach of deep reactive ion etching (DRIE) technology suitable with in situ small angle X-ray scattering characterizations. LNCs were successfully produced by a phase inversion composition (PIC) process with highly monodispersed sizes from 25 nm to 100 nm and formulated using chips manufactured by 3D printing and DRIE technologies. The transparent Si/Glass chip was also used for the small angle X-ray scattering (SAXS) analysis of the LNC formulation with the PIC process. The 3D printing and DRIE technologies and their respective advantages are discussed in terms of cost, easiness to deploy and process developments in a GALECHIP point of view. |
| Databáze: | OpenAIRE |
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