Regulations of expressions of rat/human sulfotransferases by anticancer drug, nolatrexed, and micronutrients
| Autor: | Guangping Chen, Sangita MaitiDutta, Smarajit Maiti |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
Antimetabolites Antineoplastic Cancer Research Sulfotransferase Blotting Western Leucovorin Dehydroepiandrosterone Endogeny Pharmacology Rats Sprague-Dawley chemistry.chemical_compound Folinic acid Folic Acid Sex Factors Sulfation medicine Animals Humans Pharmacology (medical) Micronutrients RNA Messenger Dose-Response Relationship Drug Chemistry Cell Cycle Hep G2 Cells Metabolism Arylsulfotransferase Rats Methotrexate Oncology Quinazolines Nolatrexed Female Sulfotransferases medicine.drug |
| Zdroj: | Anti-Cancer Drugs. 33:e525-e533 |
| ISSN: | 0959-4973 |
| Popis: | Cancer is related to the cellular proliferative state. Increase in cell-cycle regulatory function augments cellular folate pool. This pathway is therapeutically targeted. A number of drugs influences this metabolism, that is, folic acid, folinic acid, nolatrexed, and methotrexate. Our previous study showed methotrexate influences on rat/human sulfotransferases. Present study explains the effect of nolatrexed (widely used in different cancers) and some micronutrients on the expressions of rat/human sulfotransferases. Female Sprague-Dawley rats were treated with nolatrexed (01-100 mg/kg) and rats of both sexes were treated to folic acid (100, 200, or 400 mg/kg) for 2-weeks and their aryl sulfotransferase-IV (AST-IV; β-napthol sulfation) and sulfotransferase (STa; DHEA sulfation) activities, protein expression (western blot) and mRNA expression (RT-PCR) were tested. In human-cultured hepatocarcinoma (HepG2) cells nolatrexed (1 nM-1.2 mM) or folinic acid (10 nM-10 μM) were applied for 10 days. Folic acid (0-10 μM) was treated to HepG2 cells. PPST (phenol catalyzing), MPST (dopamine and monoamine), DHEAST (dehydroepiandrosterone and DHEA), and EST (estradiol sulfating) protein expressions (western-blot) were tested in HepG2 cells. Present results suggest that nolatrexed significantly increased sulfotransferases expressions in rat (protein, STa, F = 4.87, P |
| Databáze: | OpenAIRE |
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