Transcriptional inhibition of the beta-amyloid precursor protein by interferon-gamma
| Autor: | Kendra L. Burgher, Garth E. Ringheim, Jeffrey A. Heroux, Filippo Cavalieri, Ann Marie Szcepanik, Wayne Petko |
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| Rok vydání: | 1996 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide Transcription Genetic Cell Survival medicine.medical_treatment Placenta Basic fibroblast growth factor Biophysics Biology Transfection Biochemistry Cell Line chemistry.chemical_compound Amyloid beta-Protein Precursor Interferon-gamma Suppression Genetic Pregnancy mental disorders Gene expression medicine Humans Interferon gamma Luciferases Promoter Regions Genetic Molecular Biology Genomic Library Cell Biology Molecular biology Recombinant Proteins Kinetics Cytokine chemistry Cell culture Phorbol Cancer research Tetradecanoylphorbol Acetate Female Fibroblast Growth Factor 2 medicine.drug Interleukin-1 |
| Zdroj: | Biochemical and biophysical research communications. 224(1) |
| ISSN: | 0006-291X |
| Popis: | Attenuating beta-amyloid precursor protein (beta-APP) gene expression may have relevance in diseases such as Alzheimer's disease, where beta-APP has been implicated in neuropathological processes. We report here on the transcriptional down-regulation of beta-APP by interferon-gamma (IFN-gamma) in SKNMC human neuroblastoma cells. Treatment of the cells with IFN-gamma resulted in a 85% dose-dependent inhibition of beta-APP promoter activity after 24 h of exposure, with no changes observed at 5 h. For comparison, additional cytokines and signaling agents were also investigated for effects on beta-APP promoter activity. Elevated levels of activity were observed after treatment with phorbol 12-myristate 13-acetate and basic fibroblast growth factor whereas no significant effects were seen after treatment with lipopolysaccharide or interleukin-1 beta. Thus, IFN-gamma was shown here to be a suppressor of beta-APP promoter activity and is the first cytokine reported to possess such down-regulating effects. |
| Databáze: | OpenAIRE |
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