Dissecting common and divergent molecular pathways elicited by CdSe/ZnS quantum dots in freshwater and marine sentinel invertebrates
| Autor: | Mariateresa Allocca, Markus Klapper, Modi Roopin, Abuelmagd M. Abdelmonem, Wolfganag J. Parak, Oren Levy, Beatriz Pelaz, Angela Tino, Lisa-Maria Ackermann, Claudia Tortiglione, Alfredo Ambrosone, Lucia Mattera |
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| Přispěvatelé: | European Commission |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Environment health Hydra Biomedical Engineering Fresh Water 02 engineering and technology Stylophora pistillata Toxicology environment health genome–environment interactions Hydra vulgaris QD toxicity RNA-seq Transcriptome 03 medical and health sciences Anthozoa Quantum Dots Cadmium Compounds Animals 14. Life underwater Colloids Selenium Compounds Organism biology Ecology Sequence Analysis RNA Gene Expression Profiling 021001 nanoscience & nanotechnology biology.organism_classification Endocytosis genome-environment interactions Cell biology Gene expression profiling 030104 developmental biology Nanotoxicology Zinc Compounds Genome–environment interactions Lernaean Hydra 0210 nano-technology |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Nanotoxicology 11 (2017): 289–303. doi:10.1080/17435390.2017.1295111 info:cnr-pdr/source/autori:Ambrosone, Alfredo; Roopin, Modi; Pelaz, Beatriz; Abdelmonem, Abuelmagd M.; Ackermann, Lisa-Maria; Mattera, Lucia; Allocca, Mariateresa; Tino, Angela; Klapper, Markus; Parak, Wolfgang J.; Levy, Oren; Tortiglione, Claudia/titolo:Dissecting common and divergent molecular pathways elicited by CdSe%2FZnS quantum dots in freshwater and marine sentinel invertebrates/doi:10.1080%2F17435390.2017.1295111/rivista:Nanotoxicology (Print)/anno:2017/pagina_da:289/pagina_a:303/intervallo_pagine:289–303/volume:11 |
| DOI: | 10.1080/17435390.2017.1295111 |
| Popis: | Water ecosystems represent main targets of unintentional contamination of nanomaterials, due to industrial waste or other anthropogenic activities. Nanoparticle insult to living organisms may occur in a sequential way, first by chemical interactions of the material with the target membrane, then by progressive internalisation and interaction with cellular structures and organelles. These events trigger a signal transduction, through which cells modulate molecular pathway in order to respond and survive to the external elicitation. Therefore, the analysis of the global changes of the molecular machinery, possibly induced in an organism upon exposure to a given nanomaterial, may provide unique clues for proper and exhaustive risk assessment. Here, we tested the impact of core/shell CdSe/ZnS QDs coated by a positively charged polymer on two aquatic species, the polyp Hydra vulgaris and the coral S. pistillata, representative of freshwater and sea habitats, respectively. By using reliable approaches based on animal behaviour and physiology together with a whole transcriptomic profiling, we determined several toxicity endpoints. Despite the difference in the efficiency of uptake, both species were severely affected by QD treatment, resulting in dramatic morphological damages and tissue bleaching. Global transcriptional changes were also detected in both organisms, but presenting different temporal dynamics, suggesting both common and divergent functional responses in the two sentinel organisms. Due to the striking conservation of structure and genomic organisation among animals throughout evolution, our expression profiling offers new clues to identify novel molecular markers and pathways for comparative transcriptomics of nanotoxicity. This project was supported by Italy-Israel Joint Innovation Program for Scientific and Technological Cooperation in R&D to CT and by the European Commission (project FutureNanoNeeds to WJP). |
| Databáze: | OpenAIRE |
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