Lensfree diffractive tomography for the imaging of 3D cell cultures

Autor: Xavier Gidrol, J.-M. Dinten, Fabien Momey, F. Kermarrec Marcel, Anthony Berdeu, Thomas Bordy, Nathalie Picollet-D'hahan, Cédric Allier
Přispěvatelé: Laboratoire Hubert Curien / Eris, Laboratoire Hubert Curien [Saint Etienne] (LHC), Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), Institut d'Optique Graduate School (IOGS)-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), Biomicrotechnologie et génomique fonctionnelle (BIOMICS), Laboratoire de Biologie à Grande Échelle (BGE - UMR S1038), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Berdeu, Anthony, Institut d'Optique Graduate School (IOGS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Institut d'Optique Graduate School (IOGS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Hubert Curien (LHC)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
(1806900) Three-dimensional mi-croscopy References and links
Microscope
[SPI.OPTI] Engineering Sciences [physics]/Optics / Photonic
Computer science
Holography
OCIS codes: (0901970) Diffractive optics
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
01 natural sciences
Article
law.invention
010309 optics
03 medical and health sciences
Optics
law
0103 physical sciences
Microscopy
[SDV.BDD] Life Sciences [q-bio]/Development Biology
Organoid
(1700110) Imaging systems
[SDV.BC] Life Sciences [q-bio]/Cellular Biology
[SDV.BDD]Life Sciences [q-bio]/Development Biology
business.industry
Reconstruction algorithm
Atomic and Molecular Physics
and Optics
(1003010) Im-age reconstruction techniques
(0901995) Digital holography
030104 developmental biology
[SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic
Tomography
business
Phase retrieval
Digital holography
Biotechnology
Zdroj: 11ème Journées Imagerie Optique Non Conventionnelle (JIONC)
11ème Journées Imagerie Optique Non Conventionnelle (JIONC), Mar 2016, Paris, France
Biomedical optics express
Biomedical optics express, Optical Society of America-OSA Publishing, 2016, ⟨10.1364/BOE.7.000949.v008⟩
Biomedical optics express, 2016, ⟨10.1364/BOE.7.000949.v008⟩
ISSN: 2156-7085
DOI: 10.1364/BOE.7.000949.v008⟩
Popis: International audience; New microscopes are needed to help realize the full potential of 3D organoid culture studies bygathering large quantitative and systematic data over extended period of time while preserving the integrityof the living sample. On another hand, lensfree video microscopy is addressing these needs in the context of2D cell culture, providing label-free and non-phototoxic acquisition of large datasets. As scientistsroutinely adopt 3D culture techniques, the new challenging task is to extend lensfree microscopy techniquesto 3D structures.In order to image such large volume while preserving the ability to catch every single cell, we proposenew imaging platforms based on lensfree microscopy. We have built lensfree diffractive tomography setupsperforming multi-angle acquisitions of 3D organoid culture embedded in Matrigel® and developed adedicated 3D holographic reconstruction algorithm based on the Fourier diffraction theorem.With a first prototype we have been able to reconstruct a 3D volume as large as 21.5 mm^3 of a 3Dorganoid culture of prostatic RWPE1 cells in Matrigel® showing the ability of these cells to assemble in 3Dintricate cellular network at the mesoscopic scale. Comparisons with 2D images showed that it ispossible to detect single cells isolated from the main cellular structure.From results of the first prototype and numerical simulations, a second prototype wasdesigned to improve the reconstructions quality, especially on the vertical axis. First results on dust andbubbles lying at the bottom of a Petri® dish are promising.The adaptation of lensfree microscopy techniques to 3D organoid cultures imaging and thepresentation of the associated algorithms are the scopes of the present communication.
Databáze: OpenAIRE
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