Miniaturized multichannel near infrared endoscope for mouse imaging
| Autor: | Khashayarsha Khazaie, Henry S. Su, Martin Funovics, Umar Mahmood, Herlen Alencar, Ralph Weissleder |
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| Rok vydání: | 2004 |
| Předmět: |
Adenoma
Materials science lcsh:Medical technology Endoscope Biomedical Engineering Colonic Polyps Sensitivity and Specificity Endoscopic imaging Mice Optics Intestinal Neoplasms Image Processing Computer-Assisted Animals Radiology Nuclear Medicine and imaging lcsh:QH301-705.5 Endoscopes Spectroscopy Near-Infrared business.industry Near-infrared spectroscopy technology industry and agriculture Endoscopy Colonoscopy Carbocyanines Condensed Matter Physics equipment and supplies Mice Mutant Strains Disease Models Animal lcsh:Biology (General) lcsh:R855-855.5 Molecular Medicine business Software Biotechnology |
| Zdroj: | Molecular Imaging, Vol 2 (2003) |
| ISSN: | 1535-3508 |
| Popis: | We describe the design and construction of a miniaturized multichannel near infrared (NIR) endoscopic imaging system developed for high-resolution imaging of mice. The device allows for simultaneous real-time video images in white light and two independent NIR channels. Testing demonstrated independent acquisition of nanomolar concentrations of fluorochromes Cy5.5 and Cy7. Cross-talk between the NIR channels, partially a result of broad tails in the spectra of commonly used organic fluorochromes, was assessed, modeled for the linear range of the concentration/signal intensity function, and compensated. The calculated compensation was 5.5% and 22% of the total signal intensity in the two channels NIR 700 and NIR 780 , respectively, at equal concentrations of the two fluorochromes. Using a mouse model of colonic adenomatosis, we show that both perfusion and protease activity can be detected simultaneously, independently, and repeatedly in live mice. The developed device should be useful for in vivo imaging of diverse molecular targets. |
| Databáze: | OpenAIRE |
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