The Multifaceted Inhibitory Effects of an Alkylquinolone on the Diatom Phaeodactylum tricornutum

Autor: Peter G. Kroth, Dávid Szamosvári, Benjamin Bailleul, Alexandra Peltekis, Bernard Lepetit, Adrien Lapointe, Thomas Böttcher, Frederike Stock, Michaela Prothiwa, Wim Vyverman, Lachlan Dow
Přispěvatelé: University Hospital Rostock, Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Gent University, Department of Biology, University of Konstanz, Biologie du chloroplaste et perception de la lumière chez les micro-algues, Institut de biologie physico-chimique (IBPC (FR_550)), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), European Project: 715579,PhotoPHYTOMICS, Institut de biologie de l'ENS Paris (IBENS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2020
Předmět:
cytochromes
Chloroplasts
Mitochondrion
Thylakoids
01 natural sciences
Biochemistry
Adenosine Triphosphate
Marine bacteriophage
diatom–bacteria interactions
N-OXIDES
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
Plastids
ComputingMilieux_MISCELLANEOUS
MARINE ALTEROMONAS SP
Full Paper
biology
Chemistry
Full Papers
Mitochondria
3. Good health
diatom-bacteria interactions
Molecular Medicine
cytochromes diatom-bacteria interactions photosynthesis quinolone respiration
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
010402 general chemistry
Photosynthesis
PHOTOSYSTEM-II
QUINOLONES
ddc:570
Phaeodactylum tricornutum
Plastid
Molecular Biology
Diatoms
4-Quinolones
photosynthesis
QUANTUM YIELD
010405 organic chemistry
fungi
Organic Chemistry
PSEUDOMONAS-AERUGINOSA
Biology and Life Sciences
biology.organism_classification
Electron transport chain
0104 chemical sciences
reaction mechanisms
Cytochrome b6f Complex
Diatom
quinolones
Bacteria
Zdroj: ChemBioChem
ChemBioChem, Wiley-VCH Verlag, 2019, ⟨10.1002/cbic.201900612⟩
CHEMBIOCHEM
ChemBioChem, 2019, 21 (8), pp.1206-1216. ⟨10.1002/cbic.201900612⟩
Chembiochem
ISSN: 1439-7633
1439-4227
DOI: 10.1002/cbic.201900612
Popis: The mechanisms underlying interactions between diatoms and bacteria are crucial to understand diatom behaviour and proliferation, and can result in far‐reaching ecological consequences. Recently, 2‐alkyl‐4‐quinolones have been isolated from marine bacteria, both of which (the bacterium and isolated chemical) inhibited growth of microalgae, suggesting these compounds could mediate diatom–bacteria interactions. The effects of several quinolones on three diatom species have been investigated. The growth of all three was inhibited, with half‐maximal inhibitory concentrations reaching the sub‐micromolar range. By using multiple techniques, dual inhibition mechanisms were uncovered for 2‐heptyl‐4‐quinolone (HHQ) in Phaeodactylum tricornutum. Firstly, photosynthetic electron transport was obstructed, primarily through inhibition of the cytochrome b 6 f complex. Secondly, respiration was inhibited, leading to repression of ATP supply to plastids from mitochondria through organelle energy coupling. These data clearly show how HHQ could modulate diatom proliferation in marine environments.
Double trouble: In the light, 2‐heptyl‐4‐quinolone (HHQ) primarily blocks photosynthetic electron flow at the cytochrome b 6 f complex in the diatom P. tricornutum. In darkness, HHQ inhibits mitochondrial respiration, resulting in a decreased ATP supply, which ultimately leads to a decreased electric field strength in the thylakoid membranes inside the chloroplast of diatoms.
Databáze: OpenAIRE
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