Microbial and metabolic succession on common building materials under high humidity conditions
| Autor: | Christopher S. Henry, Dan Zhao, Gabriel Goodney, Brent Stephens, Erica M. Hartmann, Peng Gao, Simon Lax, Scott T. Kelley, Jack A. Gilbert, Cesar Cardona, Valerie J. Winton, Paul M. Thomas, Neil Gottel |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
16S Science Microorganism Microbial metabolism Beta diversity General Physics and Astronomy 02 engineering and technology Ecological succession Bacterial growth General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences chemistry.chemical_compound Metabolomics Microbial ecology RNA Ribosomal 16S Botany Food science lcsh:Science Phylogeny Ribosomal Multidisciplinary biology Bacteria Construction Materials Pseudomonas Biofilm Fungi Humidity General Chemistry Ribosomal RNA 021001 nanoscience & nanotechnology biology.organism_classification 030104 developmental biology Infectious Diseases chemistry Azoxystrobin Penicillium Viruses RNA Alpha diversity lcsh:Q 0210 nano-technology Infection Environmental Monitoring |
| Zdroj: | Nature communications, vol 10, iss 1 Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019) Nature Communications |
| Popis: | Despite considerable efforts to characterize the microbial ecology of the built environment, the metabolic mechanisms underpinning microbial colonization and successional dynamics remain unclear, particularly at high moisture conditions. Here, we applied bacterial/viral particle counting, qPCR, amplicon sequencing of the genes encoding 16S and ITS rRNA, and metabolomics to longitudinally characterize the ecological dynamics of four common building materials maintained at high humidity. We varied the natural inoculum provided to each material and wet half of the samples to simulate a potable water leak. Wetted materials had higher growth rates and lower alpha diversity compared to non-wetted materials, and wetting described the majority of the variance in bacterial, fungal, and metabolite structure. Inoculation location was weakly associated with bacterial and fungal beta diversity. Material type influenced bacterial and viral particle abundance and bacterial and metabolic (but not fungal) diversity. Metabolites indicative of microbial activity were identified, and they too differed by material. Microbes inhabit built environments and could contribute to degradation of surfaces especially in damp conditions. Here the authors explore how communities of microbes and their metabolites affect four types of built surfaces under varying environmental conditions. |
| Databáze: | OpenAIRE |
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