Species Restriction of a Monoclonal Antibody Reacting with Residues 130 to 137 in Encephalitogenic Myelin Basic Protein
| Autor: | Marian W. Kies, Russell E. Martenson, E. D. Beckman, Ingegerd Hellström, Karl Erik Hellström, J. E. Casnellie, G. E. Deibler, L. R. Sires, Sarka Hruby, Ellsworth C. Alvord |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
medicine.drug_class
Guinea Pigs Peptide Monoclonal antibody Epitopes Species Specificity Thermolysin medicine Animals Humans Amino Acid Sequence Binding site chemistry.chemical_classification Multidisciplinary biology Chemistry Antibodies Monoclonal Myelin Basic Protein Molecular biology Peptide Fragments Rats Myelin basic protein Amino acid Biochemistry biology.protein Macaca Cattle Rabbits Antibody Digestion Chickens |
| Zdroj: | Science. 214:87-89 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.6169147 |
| Popis: | A monoclonal antibody (immunoglobulin G1) has been produced that reacts against myelin basic protein present in or extracted from the brains of many mammals-with certain important exceptions. Because of known species differences in amino acid sequences of basic protein and of certain peptide fragments, the binding site for this particular antibody appeared likely to include residues 130 to 137. Confirmation of this hypothesis was obtained by amino acid composition of the major immunoreactive peptides produced by thermolysin digestion of human basic protein and isolated by high-performance liquid chromatography. |
| Databáze: | OpenAIRE |
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