Analysis of serum Haptoglobin using glycoproteomics and lectin immunoassay in liver diseases in Hepatitis B virus infection
| Autor: | A. Shukla, Bhavik Dalal, K. Dalal, S. Bhatia, Aruna Shankarkumar |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Proteomics 0301 basic medicine Carcinoma Hepatocellular Glycosylation Cirrhosis Clinical Biochemistry medicine.disease_cause Biochemistry 03 medical and health sciences 0302 clinical medicine Tandem Mass Spectrometry Lectins Biomarkers Tumor medicine Humans Fucosylation Fucose Glycoproteins Immunoassay Hepatitis B virus Haptoglobins medicine.diagnostic_test biology business.industry Liver Diseases Liver Neoplasms Biochemistry (medical) Haptoglobin Lectin Blood Proteins General Medicine Middle Aged Hepatitis B medicine.disease digestive system diseases Glycoproteomics 030104 developmental biology 030220 oncology & carcinogenesis Hepatocellular carcinoma Immunology biology.protein Female business Chromatography Liquid |
| Zdroj: | Clinica Chimica Acta. 495:309-317 |
| ISSN: | 0009-8981 |
| Popis: | Hepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is a major cause in Asia-Pacific countries. Its early detection is of paramount importance using a marker having both sensitivity and specificity. The present study promises diagnostic and prognostic markers by the identification of site-specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.Three groups of patients: chronic, liver cirrhosis and HCC with HBV infection along with controls were enrolled. Hp was purified using affinity column chromatography and, peptide sequence, N-glycosylation site, glycan composition and glycoforms were identified using mass spectrometry. Quantitative lectin ELISA was used to measure levels of fucosylation on Hp in liver diseases due to HBV.Hp levels were significantly lower in HCC when compared with Non-HCC cases (p .05). Fucosylated glycoforms were significantly increased at site Asn184, Asn207 and Asn211 in liver diseased stages versus controls. A significant association was observed between the Fuc-Hp/Hp Elisa index and, advanced liver disease stages and controls using lectin Elisa (p .001).Quantitation of fucosylation levels on Hp protein using Lectin ELISA may be useful glycobiomarker either alone or in combination (AFP + DCP + FucHp; AUC = 0.94) in HBV HCC diagnosis in clinical practice. |
| Databáze: | OpenAIRE |
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