Heterologous expression of a putative ClpC chaperone gene leads to induction of a host metabolite
| Autor: | Jana Braesel, Alessandra S. Eustáquio |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Regulation of gene expression
Cloning natural product Chaperone Gene gene activation 010401 analytical chemistry Streptomyces coelicolor General Chemistry Computational biology Clp chaperone Biology biology.organism_classification 01 natural sciences DNA sequencing Article 0104 chemical sciences Synthetic biology antibiotic Clp protease Clp ATPase genome mining Heterologous expression Gene |
| Zdroj: | J Braz Chem Soc Journal of the Brazilian Chemical Society, Volume: 30, Issue: 3, Pages: 499-508, Published: MAR 2019 Journal of the Brazilian Chemical Society v.30 n.3 2019 Journal of the Brazilian Chemical Society Sociedade Brasileira de Química (SBQ) instacron:SBQ |
| ISSN: | 0103-5053 |
| Popis: | Genome mining provides exciting opportunities for the discovery of natural products. However, in contrast to traditional bioassay-guided approaches, challenges of genome mining include poor or no expression of biosynthetic gene clusters (BGCs). Additionally, given that thousands of BGCs are now available through extensive genome sequencing, how does one select BGCs for discovery? Synthetic biology techniques can be used for BGC refactoring and activation, whereas resistance-gene-directed genome mining is a promising approach to discover bioactive natural products. Here we report the selection of a BGC by applying a resistance-gene-directed approach, cloning of the silent BGC from Micromonospora sp. B006, promoter exchange, and heterologous expression in Streptomyces coelicolor M1152. While we have yet to identify the encoded compound, we unexpectedly observed induction of a host metabolite, which we hypothesize is due to the presence of a caseinolytic protease C (ClpC) chaperone gene in the BGC, suggesting that ClpC chaperones may be used for BGC activation. |
| Databáze: | OpenAIRE |
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