Intracellular distribution and substrate specificity of the 16α-hydroxylase in the adrenal of human fetus
| Autor: | Shimizu Kyutaro, Yamasaki Hiroko |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
medicine.medical_specialty
Chromatography Paper Metabolite Clinical Biochemistry Dehydroepiandrosterone Tritium Biochemistry chemistry.chemical_compound Cytosol Fetus Endocrinology Pregnancy Microsomes Internal medicine Adrenal Glands medicine Humans Carbon Radioisotopes Androstenedione Molecular Biology Incubation Progesterone Cell Nucleus Pharmacology Chemistry Organic Chemistry Mitochondria Pregnenolone Steroid Hydroxylases Microsome Female Chromatography Thin Layer Intracellular Subcellular Fractions medicine.drug |
| Zdroj: | Steroids. 22:637-658 |
| ISSN: | 0039-128X |
| DOI: | 10.1016/0039-128x(73)90112-8 |
| Popis: | When [7α-3H] dehydroepiandrosterone was incubated with the adrenal homogenates of human fetus at 22 to 26 weeks gestational age, 16α-hydroxydehydroepiandrosterone and/or its sulfate was formed as the only detectable metabolite. The 16α-hydroxylase activity was concentrated in the microsomal fraction of the adrenal homogenate. [1,2-3H]Androstenedione, [4-14C] pregnenolone and [7α-3H] progesterone were also 16α-hydroxylated by incubation with the microsomal fraction. Amoung these substrates, progesterone gave the highest yield of 16α-hydroxylated products. By incubation with the microsomal fraction, formation of following steroids were also established: 6β-hydroxyandrostenedione from androstenedione; 17-hydroxypregnenolone, 17,21-dihydroxypregnenolone and dehydroepiandrosterone from pregnenolone; 17-hydroxy-progesterone, deoxycorticosterone, 11-deoxycortisol and androstenedione from progesterone. |
| Databáze: | OpenAIRE |
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