Opposing roles for neurotrophin-3 in targeting and collateral formation of distinct sets of developing cortical neurons
| Autor: | Jürgen Bolz, Valérie Castellani |
|---|---|
| Přispěvatelé: | Centre de génétique et de physiologie moléculaire et cellulaire (CGPhiMC), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon |
| Rok vydání: | 1999 |
| Předmět: |
MESH: Signal Transduction
MESH: Axons MESH: Rats MESH: Neurons [SDV.BC]Life Sciences [q-bio]/Cellular Biology Neurotrophin-3 Receptors Nerve Growth Factor Tropomyosin receptor kinase C MESH: Receptors Nerve Growth Factor 03 medical and health sciences 0302 clinical medicine Neurotrophin 3 Culture Techniques medicine Animals MESH: Animals Receptor trkC Nerve Growth Factors Receptor Molecular Biology 030304 developmental biology Cerebral Cortex Neurons 0303 health sciences biology MESH: Nerve Growth Factors MESH: Receptor trkC Receptor Protein-Tyrosine Kinases Cell migration Anatomy Embryonic stem cell MESH: Cerebral Cortex Axons MESH: Neurotrophin 3 Rats medicine.anatomical_structure Nerve growth factor MESH: Culture Techniques nervous system Cerebral cortex biology.protein MESH: Receptor Protein-Tyrosine Kinases Signal transduction Neuroscience 030217 neurology & neurosurgery Developmental Biology Signal Transduction |
| Zdroj: | Development (Cambridge, England) Development (Cambridge, England), Company of Biologists, 1999, 126 (15), pp.3335-45 ResearcherID |
| ISSN: | 0950-1991 1477-9129 |
| Popis: | Neurotrophin-3 and its receptor TrkC are expressed during the development of the mammalian cerebral cortex. To examine whether neurotrophin-3 might play a role in the elaboration of layer-specific cortical circuits, slices of layer 6 and layers 2/3 neurons were cultured in the presence of exogenously applied neurotrophin-3. Results indicate that neurotrophin-3 promotes axonal branching of layer 6 axons, which target neurotrophin-3-expressing layers in vivo, and that it inhibits branching of layers 2/3 axons, which avoid neurotrophin-3-expressing layers. Such opposing effects of neurotrophin-3 on axonal branching were also observed with embryonic cortical neurons, indicating that the response to neurotrophin-3 is specified at early developmental stages, prior to cell migration. In addition to its effects on fiber branching, axonal guidance assays also indicate that neurotrophin-3 is an attractive signal for layer 6 axons and a repellent guidance cue for layers 2/3 axons. Experiments with specific antibodies to neutralize neurotrophin-3 in cortical membranes revealed that endogenous levels of neurotrophin-3 are sufficient to regulate branching and targeting of cortical axons. These opposing effects of neurotrophin-3 on specific populations of axons demonstrate that it could serve as one of the signals for the elaboration of local cortical circuits. |
| Databáze: | OpenAIRE |
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